Skip to main content

Mouse/Rat SIRP alpha/CD172a Alexa Fluor® 488-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7307G

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse, Rat

Applications

Flow Cytometry

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 772734

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat SIRP alpha/CD172a
Lys32-Asn373
Accession # P97710

Specificity

Detects rat SIRP alpha/CD172a in direct ELISAs and Western blots. Detects mouse and rat SIRP alpha/CD172a in flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images

Detection of SIRPa/CD172a antibody in PC-12 Rat Cell Line antibody by Flow Cytometry.

Detection of SIRP alpha/CD172a in PC‑12 Rat Cell Line by Flow Cytometry.

PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRPa/CD172a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7307G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of SIRPa/CD172a antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of SIRP alpha/CD172a in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b APC-conjugated Monoclonal Antibody (Catalog # FAB1124A) and either (A) Mouse Anti-Mouse/Rat SIRPa/CD172a Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB7307G) or (B) Mouse IgG2BAlexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.

Applications

Application
Recommended Usage

Flow Cytometry

5 µL/106 cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line and mouse splenocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: SIRP alpha/CD172a

SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Entrez Gene IDs

140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)

Gene Symbol

SIRPA

UniProt

Additional SIRP alpha/CD172a Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Loading...
Loading...
Loading...
Loading...
Loading...