PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Mouse/Rat SIRPa/CD172a APC-conjugated Monoclonal Antibody (Catalog # FAB7307A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of SIRP alpha/CD172a in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Mouse Integrin aM/CD11b PE-conjugated Monoclonal Antibody (Catalog # FAB1124P) and either (A) Mouse Anti-Mouse/Rat SIRPa/CD172a APC-conjugated Monoclonal Antibody (Catalog # FAB7307A) or (B) Mouse IgG_2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse/Rat SIRP alpha/CD172a APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: PC‑12 rat adrenal pheochromocytoma cell line and mouse splenocytes

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: SIRP alpha/CD172a

SIRP alpha (Signal regulatory protein alpha; also known as CD172a, Shps1 and Bit) is a variably glycosylated, 85-120 kDa member of the SIRP ‘family' of proteins. It is expressed on neurons, macrophages, monocytes, granulocytes and dendritic cells. SIRP alpha is phosphorylated/activated in response to cell adhesion. This may, or may not, involve binding to known ligands CD47, SP-A and SP-D. Following phosphorylation, SIRP alpha binds to SHP-1 and -2, resulting in the negative regulation of immune system activity. Mature rat SIRP alpha is a 478 amino acid (aa) type I transmembrane glycoprotein. It contains a 342 aa extracellular region (aa 32-373) that possesses one V-type and two C1-type Ig-like domains. Its cytoplasmic domain possesses two ITIMs that interact with protein phosphatases. There is one potential splice variant that shows a four aa insertion after Gln424. Over aa 32-373, rat SIRP alpha shares 63% and 73% aa sequence identity with human and mouse SIRP alpha, respectively.

Long Name

Signal-regulatory Protein alpha

Alternate Names

BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA

Entrez Gene IDs

140885 (Human); 19261 (Mouse); 25528 (Rat); 494566 (Porcine); 609452 (Canine); 101926317 (Cynomolgus Monkey)

Gene Symbol

SIRPA

UniProt

P97710

Additional SIRP alpha/CD172a Products

Product Documents for Mouse/Rat SIRP alpha/CD172a APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Mouse/Rat SIRP alpha/CD172a APC-conjugated Antibody

For research use only

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Flow Cytometry

Mouse/Rat SIRP alpha/CD172a APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB7307A

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Mouse/Rat SIRP alpha/CD172a APC-conjugated Antibody

Detection of SIRP alpha/CD172a in PC‑12 Rat Cell Line by Flow Cytometry.