Numb Antibody [DyLight 405]

Novus Biologicals, part of Bio-Techne | Catalog # NB500-178V

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Predicted:

Rat (90%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Numb Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region of mouse NUMB (between residues 600-653). [UniProt# Q9QZS3]

Reactivity Notes

Immunogen sequence has 90% identity to rat.

Localization

Membrane-associated

Specificity

This is specific for all four isoforms of the NUMB protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Numb Antibody [DyLight 405]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Numb

Numb is an evolutionary conserved protein which plays a crucial role in cell fate determination, an important event in embryonic development as well as in certain pathologies including cancer. Numb was initially identified in Drosophila (dNumb) as a membrane-associated protein which asymmetrically segregates in dividing cells and define distinct cell fates by interacting with/inhibiting Notch. dNumb's mammalian homologs (encoded by two genes mNumb and mNumb-like) displays a higher degree of complexity with alternative spliced isoforms, and display multitude of functions such as control of asymmetric cell division and cell fate choice, endocytosis, cell adhesion, cell migration, ubiquitination of specific substrates and regulation of Notch, Hedgehog, p53 etc. The multiple functions of Numb highlight its critical role not only in the control of physiological processes, but also in diseases, when deregulated. The ability of Numb to control oncogenic (Notch and Hedgehog) and tumor suppressor (p53) pathways provides a strong link between deregulated Numb function and cancer. Indeed, hyperactive Notch and Hedgehog functions have been described to be responsible of several tumors including breast cancer and medulloblastoma.

Alternate Names

m-numb, mnb, S171

Gene Symbol

NUMB

Additional Numb Products

Product Documents for Numb Antibody [DyLight 405]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Numb Antibody [DyLight 405]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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