PAR/pADPr Antibody Best Seller

Detection of Human PAR/pADPr by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 2 mM H₂O₂ for 5 minutes. PVDF membrane was probed with 1:1000 dilution of Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for PAR polymers, as indicated. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of PAR in Jurkat cells by Flow Cytometry.

Jurkat human acute T cell leukemia cell line treated with 1 mM H₂O₂ for 5 minutes (filled histogram) or resting (open histogram) were stained with Mouse Anti-PAR Monoclonal Antibody (Catalog # 4335-MC-100) followed by Goat anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized using FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Detection of PAR/pADPr in Human Kidney.

PAR/pADPr was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-PAR/pADPr Monoclonal Antibody (Catalog # 4335-MC-100) at 1.7 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.