Rat Adiponectin/Acrp30 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3100
Key Product Details
Species Reactivity
Validated:
Rat
Cited:
Rat
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 861812
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Adiponectin/Acrp30
Met1-Asn244
Accession # NP_653345
Met1-Asn244
Accession # NP_653345
Specificity
Detects rat Adiponectin/Acrp30 in ELISAs. In direct ELISAs, no cross-reactivity with recombinant human or recombinant mouse Adiponectin is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Rat Adiponectin/Acrp30 Antibody
Detection of Rat Adiponectin/Acrp30 by Western Blot.
Western blot shows lysates of rat adipose tissue and rat mammary gland tissue. PVDF membrane was probed with 0.25 µg/mL of Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Adiponectin/Acrp30 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Adiponectin/Acrp30 in Rat Adipocytes.
Adiponectin/Acrp30 was detected in Immersion fixed rat mesenchymal stem cells differentiated into adipocytes with Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011) using Mouse Anti-Rat Adiponectin/Acrp30 Monoclonal Antibody (Catalog # MAB3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Rat Adiponectin/Acrp30 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011)
Sample: Immersion fixed rat mesenchymal stem cells differentiated to adipocytes using Human/Mouse/Rat StemXVivo Adipogenic Supplement (Catalog # CCM011)
Western Blot
1 µg/mL
Sample: Rat adipose tissue and rat mammary gland tissue
Sample: Rat adipose tissue and rat mammary gland tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB3100 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Adiponectin/Acrp30
Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.
Alternate Names
Acrp30, AdipoQ, ApM1, GBP28
Gene Symbol
ADIPOQ
UniProt
Additional Adiponectin/Acrp30 Products
Product Documents for Rat Adiponectin/Acrp30 Antibody
Product Specific Notices for Rat Adiponectin/Acrp30 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...