RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49546

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Chicken, Drosophila, Yeast

Cited:

Human, Insect - Drosophila, Yeast

Applications

Validated:

Dot Blot, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.1 mg/ml

View all RNA Polymerase II/POLR2A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]

Modification

p Thr4

Localization

Nucleus.

Specificity

This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

Western Blot: RNA Polymerase II/POLR2A [p Thr4] AntibodyBSA Free [NBP1-49546]

Western Blot: RNA Polymerase II/POLR2A [p Thr4] AntibodyBSA Free [NBP1-49546]

Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Western Blot analysis of RNA Pol II (pThr 4) in 293T cells. Image submitted via verified customer review.
Western Blot: RNA Polymerase II/POLR2A [p Thr4] AntibodyBSA Free [NBP1-49546]

Western Blot: RNA Polymerase II/POLR2A [p Thr4] AntibodyBSA Free [NBP1-49546]

Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Analysis of RNA pol II CTD pT4 in A. DT40 cell lysate and B. HeLa cell lysate.
Western Blot: RNA Polymerase II/POLR2A [p Thr4] AntibodyBSA Free [NBP1-49546]

Western Blot: RNA Polymerase II/POLR2A [p Thr4] AntibodyBSA Free [NBP1-49546]

Western Blot: RNA Polymerase II/POLR2A [p Thr4] Antibody [NBP1-49546] - Flag-tagged Rpb1 was IP'd with Flag antibody. Western blotting was performed using P-thr4 antibody. 26r: Rpb1 with 26 all-consensus YSPTSPS repeat CTD. T4V: Rpb1 with 30 YSPVSPS repeat CTD.
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Applications for RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

Application
Recommended Usage

Dot Blot

1:1000

Western Blot

1:1000
Application Notes
This RNA polymerase II antibody is useful for dot blot and Western blot.

Reviewed Applications

Read 1 review rated 5 using NBP1-49546 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is a 12-subunit enzyme which synthesizes all mRNAs as well as several non-coding RNAs in eukaryotic cells thereby playing a central role in cellular functions. RNAPII is a DNA-dependent RNA polymerase enzyme which catalyzes the transcription of DNA into RNA employing four ribonucleoside triphosphates as substrates. The activity of RNAPII transcription machinery is regulated by DNA-binding transcriptional regulators in a gene/cell type-specific manner, whereas general transcription factors act as RNAPII accessory proteins required for the transcription of most class II genes, and the co-activators/co-repressors serve as bridges between DNA-bound factors and the RNAPII machinery, some affecting the organization and/or chemical modification of the chromatin template of RNAPII. RNA polymerase II subunit B1 (POLR2A) is the largest and the catalytic component of RNAPII which forms the polymerase active center together with the second largest subunit and is the central component of the basal RNAPII transcription machinery. It is composed of mobile elements that move relative to each other and POLR2A is the part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Entrez Gene IDs

5430 (Human)

Gene Symbol

POLR2A

UniProt

P24928 (Human)

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A [p Thr4] Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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