RNA Polymerase II/POLR2A [p Thr4] Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49546CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Chicken, Drosophila, Yeast
Applications
Dot Blot, Western Blot
Label
CoraFluor 1
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]
Modification
p Thr4
Localization
Nucleus.
Specificity
This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for RNA Polymerase II/POLR2A [p Thr4] Antibody [CoraFluor™ 1]
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: RNA Polymerase II/POLR2A
Long Name
DNA-directed RNA Polymerase II Subunit RPB1
Alternate Names
POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2
Gene Symbol
POLR2A
Additional RNA Polymerase II/POLR2A Products
Product Documents for RNA Polymerase II/POLR2A [p Thr4] Antibody [CoraFluor™ 1]
Product Specific Notices for RNA Polymerase II/POLR2A [p Thr4] Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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