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RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 647]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-49546AF647

Novus Biologicals, part of Bio-Techne
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NBP1-49546AF647

Key Product Details

Species Reactivity

Human, Chicken, Drosophila, Yeast

Applications

Dot Blot, Western Blot

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide made to a phosphorylated Threonine (position 4) of the CTD heptad in the human RNA polymerase II protein [UniProt P24928]

Modification

p Thr4

Localization

Nucleus.

Specificity

This antibody is specific for the phosphorylated Threonine found in position 4 of the RNA polymerase II CTD heptad.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 647]

Application
Recommended Usage

Dot Blot

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for RNA Polymerase II/POLR2A [p Thr4] Antibody [Alexa Fluor® 647]

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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