Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51641APC
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate, Rabbit
Applications
ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 1F3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin]
Immunogen
Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Reactivity Notes
Use in Rabbit reported in scientific literature (PMID:32179074).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin]
Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin] [NBP1-51641APC] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
Applications for Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Sirtuin 1/SIRT1
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1
Gene Symbol
SIRT1
Additional Sirtuin 1/SIRT1 Products
Product Documents for Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin]
Product Specific Notices for Sirtuin 1/SIRT1 Antibody (1F3) [Allophycocyanin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...