Sirtuin 1/SIRT1 Antibody (1F3) [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-51641CL1
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Primate, Rabbit
Applications
ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Monoclonal Mouse IgG1 Clone # 1F3
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Sirtuin 1/SIRT1 Antibody (1F3) [CoraFluor™ 1]
Immunogen
Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]
Reactivity Notes
Use in Rabbit reported in scientific literature (PMID:32179074).
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Sirtuin 1/SIRT1 Antibody (1F3) [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow (Intracellular)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry Free-Floating
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Sirtuin 1/SIRT1
Long Name
Silent Mating Type Information Regulation 2 Homolog
Alternate Names
SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1
Gene Symbol
SIRT1
Additional Sirtuin 1/SIRT1 Products
Product Documents for Sirtuin 1/SIRT1 Antibody (1F3) [CoraFluor™ 1]
Product Specific Notices for Sirtuin 1/SIRT1 Antibody (1F3) [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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