Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80949

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate, Rabbit

Applications

CyTOF-ready, ELISA, Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1F3

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Sirtuin 1/SIRT1 Antibodies »

Product Summary for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

Immunogen

Purified recombinant fragment of human SIRT1 (amino acids: 265-452) expressed in E. coli. [UniProt# Q96EB6]

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

Western Blot: Sirtuin 1/SIRT1 Antibody (1F3)Azide and BSA Free [NBP2-80949]

Western Blot: Sirtuin 1/SIRT1 Antibody (1F3)Azide and BSA Free [NBP2-80949]

Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]

Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]

Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. Image from the standard format of this antibody.
Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]

Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949]

Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free [NBP2-80949] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining. Image from the standard format of this antibody.
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Applications for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 26327687)

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sirtuin 1/SIRT1

SIRT1, the human homolog of the S. cerevisiae SIR2 protein, functions as an NAD-dependent deacetylase of a number of nonhistone substrates including p53. In response to DNA damage, SIRT1 binds and deacetylates the p53 protein at c-terminal Lys382 residue and attenuate p53-mediated functions. When overexpressed in mouse embryo fibroblasts, SIRT1 antagonizes PML-induced acetylation of p53 and rescues PML-mediated premature cellular senescence. In mammalian cells, SIRT1 appears to control the cellular response to stress by regulating the FOXO family of forkhead transcription factors.

Long Name

Silent Mating Type Information Regulation 2 Homolog

Alternate Names

SIR2-like protein 1, SIR2alpha, SIR2L1, SIRT1

Gene Symbol

SIRT1

Additional Sirtuin 1/SIRT1 Products

Product Documents for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Sirtuin 1/SIRT1 Antibody (1F3) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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