TIMP-2 Antibody (TIMP2/8193R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20229
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # TIMP2/8193R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant fragment of human TIMP-2 protein (exact sequence is proprietary)
Localization
Cytoplasm. Cell surface.
Specificity
It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2) It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP-2 or gelatinase A) It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP-9 or gelatinase B) TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Normal Placenta, Prostate or Ovarian Carcinoma.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for TIMP-2 Antibody (TIMP2/8193R)
TIMP-2 Antibody (TIMP2/8193R)
IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using TIMP-2 antibody (TIMP2/8193R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
TIMP-2 Antibody (TIMP2/8193R)
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with TIMP-2 antibody (TIMP2/8193R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.Applications for TIMP-2 Antibody (TIMP2/8193R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: TIMP-2
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
Additional TIMP-2 Products
Product Documents for TIMP-2 Antibody (TIMP2/8193R)
Product Specific Notices for TIMP-2 Antibody (TIMP2/8193R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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