TIMP-3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29478

Novus Biologicals, part of Bio-Techne
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NBP2-29478
NBP2-29478-20ug
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Equine

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all TIMP-3 Antibodies »

Product Summary for TIMP-3 Antibody

Immunogen

TIMP-3 (aa175-211) (Uniprot: P35625)

Reactivity Notes

The amino acid sequence used as the immunogen for the TIMP3 antibody is 100% homologous in human, cow, dog and horse, and 94% homologous in mouse and rat. Sheep data from customer review.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

30 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33098)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TIMP-3 Antibody

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478] - TIMP-3 is expressed in first trimester human decidual cells. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478]

Immunohistochemistry-Paraffin: TIMP-3 Antibody [NBP2-29478] - Formalin-fixedn, paraffin-embedded human placenta stained with TIMP3 Rabbit polyclonal antibody

Applications for TIMP-3 Antibody

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 3.7 using NBP2-29478 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TIMP-3

TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP's inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Alternate Names

TIMP3

Gene Symbol

TIMP3

UniProt

P35625

Additional TIMP-3 Products

Product Documents for TIMP-3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for TIMP-3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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