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TIMP-3 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33098

Novus Biologicals, part of Bio-Techne
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NBP2-33098

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Equine

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

1.0 mg/ml

Product Summary for TIMP-3 Antibody - Azide and BSA Free

Immunogen

TIMP-3 (aa175-211) (Uniprot: P35625)

Reactivity Notes

The amino acid sequence used as the immunogen for the TIMP3 antibody is 100% homologous in human, cow, dog and horse, and 94% homologous in mouse and rat. Sheep data from customer review.

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

1.0 mg/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP2-29478).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for TIMP-3 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: TIMP-3 Antibody - Azide and BSA Free [NBP2-33098]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - Azide and BSA Free [NBP2-33098]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - Azide and BSA Free [NBP2-33098] - TIMP-3 is expressed in first trimester human decidual cells. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: TIMP-3 Antibody - Azide and BSA Free [NBP2-33098]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - Azide and BSA Free [NBP2-33098]

Immunohistochemistry-Paraffin: TIMP-3 Antibody - Azide and BSA Free [NBP2-33098] - Formalin-fixed, paraffin-embedded human placenta stained with TIMP-3 Rabbit PAb.

Applications for TIMP-3 Antibody - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

0.5-1ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2ug/mlimmunohistochemistry 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml

Immunohistochemistry

0.5-1ug/ml

Immunohistochemistry-Paraffin

0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: TIMP-3

TIMPs form tight inhibitory complexes with MMPs, which are involved in matrix degradation and in tumor invasiveness and metastasis. TIMPs inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Alternate Names

TIMP3

Gene Symbol

TIMP3

Additional TIMP-3 Products

Product Documents for TIMP-3 Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for TIMP-3 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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