Ubiquitin Antibody (Ubi-1)

Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - FFPE section of cerebral cortex of an Alzheimer patient processed with Ubiquitin antibody using HRP/DAB (Brown) and also stained with haemotoxylin (Blue). A typical flame shaped tangle is seen in a pyramidal neuron in the center and is surrounded by some dystrophic neurites, also strongly ubiquitin positive. Both are commonly seen in cortical and hippocampal Alzheimer brain sections and are typical for this disease, but are rare or absent in healthy brain.

Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - Staining of Ubiquitin in hippocampal tissue from an Alzheimer patient.

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130]

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Molecular Analysis of XopD Ub/Ubl Specificity. S. lycopersicum protein extract blotted for total Ub (Ubiquitin; Novus Biologicals) following a 1 hr room temperature treatment with the XopD variants used in (K) at 1 uM final concentration. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27425412/) licensed under a CC-BY license.

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130]

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - Western blot of Ubiquitin expression on (Lane 1) poly-ubiquitin (lys63 linked), (Lane 2) pure ubiquitin and crude homogenates of adult rat, (Lane 3) cortex, (Lane 4) cerebellum and (Lane 5) brain stem.

Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130]

Immunohistochemistry-Paraffin: Ubiquitin Antibody (Ubi-1) [NB300-130] - IHC staining of Ubiquitin in human rectal cancer using DAB with hematoxylin counterstain.

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] -

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - In Vivo Applications of Affimers(A) HA-NleL 293 T-Rex cells were induced with 1 μg/mL doxycycline for 12 hr or left untreated. Whole-cell lysate (WCL) blots are shown for actin, HA(-NleL), Ub, & K6 chains. Western blots with the K6 affimer are also shown after Ub enrichment using TUBEs.(B) TUBE-PD of HEK293 cells after 1 hr of MG132 (10 μM) without further treatment (−) or with additional UV (40 J/m2) or CCCP treatment (10 μM for 1 hr) & subsequently blotted with the K6 affimer. Input controls are shown for total Ub & actin & gammaH2AX. The relative signal increase from two experiments is shown below the respective lanes.(C) Expression of WT or catalytically inactive (C431S) Parkin in HeLa Flp-In cells was induced with 0.2 μg/mL doxycycline for 16 hr. Mitochondria were depolarized with O/A for 2 hr. WCL inputs are shown for total Ub, expressed Parkin, TOM20, & actin. The TUBE-PD was also blotted using the K6 affimer & total Ub.(D) Confocal fluorescence microscopy images of cells as in (B) stained with K6 affimer (green), TOM20 (red), & DAPI (blue). Cells & a magnified area are outlined in white. Scale bars correspond to 20 μm.See also Figure S5. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] -

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - In Vivo Applications of Affimers(A) HA-NleL 293 T-Rex cells were induced with 1 μg/mL doxycycline for 12 hr or left untreated. Whole-cell lysate (WCL) blots are shown for actin, HA(-NleL), Ub, & K6 chains. Western blots with the K6 affimer are also shown after Ub enrichment using TUBEs.(B) TUBE-PD of HEK293 cells after 1 hr of MG132 (10 μM) without further treatment (−) or with additional UV (40 J/m2) or CCCP treatment (10 μM for 1 hr) & subsequently blotted with the K6 affimer. Input controls are shown for total Ub & actin & gammaH2AX. The relative signal increase from two experiments is shown below the respective lanes.(C) Expression of WT or catalytically inactive (C431S) Parkin in HeLa Flp-In cells was induced with 0.2 μg/mL doxycycline for 16 hr. Mitochondria were depolarized with O/A for 2 hr. WCL inputs are shown for total Ub, expressed Parkin, TOM20, & actin. The TUBE-PD was also blotted using the K6 affimer & total Ub.(D) Confocal fluorescence microscopy images of cells as in (B) stained with K6 affimer (green), TOM20 (red), & DAPI (blue). Cells & a magnified area are outlined in white. Scale bars correspond to 20 μm.See also Figure S5. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] -

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - HUWE1 Assembles K6 Chains In Vitro & In Vivo(A) Table summarizing proteins identified with the corresponding number peptide-spectrum matches (PSMs) in three replicates of DUB-treated K6 affimer pull-downs.(B) In vitro assembly reaction of the HECT E3 HUWE1 with Ub WT, Ub K6R, Ub K11R, & Ub K48R on Coomassie. Arrows indicate K6 diUb.(C) Linkage composition of HUWE1-generated diUb after 1 hr as determined by AQUA MS.(D) AQUA-derived total cellular chain composition of HUWE1−/− HeLa & parental cells after TUBE-based enrichment. Error bars indicate mean ± SD from n = 3. ∗p < 0.05, according to a two-tailed Student’s t test. N.S., not significant.(E) TUBE-PD from a doxycycline-inducible HUWE1 shRNA Ls174T cell line blotted with the K6-specific affimer, with input controls for actin, total Ub, & HUWE1.(F) K6 affimer pull-down in doxycycline-inducible HUWE1 shRNA Ls174T cells blotted against Mfn2. Cells were left untreated or treated with 10 μg/mL MG132 for 4 hr and/or 1 μg/mL doxycycline for 72 hr. Pull-downs were incubated with 250 nM USP21 as indicated, with a K48 blot to show completeness of the deubiquitination reaction. Input controls are shown for Mfn2, actin, & HUWE1.See also Figure S7. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] -

Western Blot: Ubiquitin Antibody (Ubi-1) [NB300-130] - In Vivo Applications of Affimers(A) HA-NleL 293 T-Rex cells were induced with 1 μg/mL doxycycline for 12 hr or left untreated. Whole-cell lysate (WCL) blots are shown for actin, HA(-NleL), Ub, & K6 chains. Western blots with the K6 affimer are also shown after Ub enrichment using TUBEs.(B) TUBE-PD of HEK293 cells after 1 hr of MG132 (10 μM) without further treatment (−) or with additional UV (40 J/m2) or CCCP treatment (10 μM for 1 hr) & subsequently blotted with the K6 affimer. Input controls are shown for total Ub & actin & gammaH2AX. The relative signal increase from two experiments is shown below the respective lanes.(C) Expression of WT or catalytically inactive (C431S) Parkin in HeLa Flp-In cells was induced with 0.2 μg/mL doxycycline for 16 hr. Mitochondria were depolarized with O/A for 2 hr. WCL inputs are shown for total Ub, expressed Parkin, TOM20, & actin. The TUBE-PD was also blotted using the K6 affimer & total Ub.(D) Confocal fluorescence microscopy images of cells as in (B) stained with K6 affimer (green), TOM20 (red), & DAPI (blue). Cells & a magnified area are outlined in white. Scale bars correspond to 20 μm.See also Figure S5. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28943312), licensed under a CC-BY license. Not internally tested by Novus Biologicals.