Ubiquitin Antibody (Ubi-1) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-130CL1

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Product Datasheet / COA / SDS

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB300-130

Alexa Fluor 350

NB300-130AF350

Alexa Fluor 405

NB300-130AF405

Alexa Fluor 488

NB300-130AF488

Alexa Fluor 532

NB300-130AF532

Alexa Fluor 594

NB300-130AF594

Alexa Fluor 647

NB300-130AF647

Alexa Fluor 700

NB300-130AF700

Alexa Fluor 750

NB300-130AF750

Allophycocyanin

NB300-130APC

Biotin

NB300-130B

DyLight 350

NB300-130UV

DyLight 405

NB300-130V

DyLight 488

NB300-130G

DyLight 550

NB300-130R

DyLight 594

NB300-130DL594

DyLight 650

NB300-130C

DyLight 680

NB300-130FR

DyLight 755

NB300-130IR

FITC

NB300-130F

HRP

NB300-130H

Janelia Fluor 525

NB300-130JF525

Janelia Fluor 549

NB300-130JF549

Janelia Fluor 585

NB300-130JF585

Janelia Fluor 635

NB300-130JF635

Janelia Fluor 646

NB300-130JF646

Janelia Fluor 669

NB300-130JF669

mFluor Violet 450 SE

NB300-130MFV450

mFluor Violet 500 SE

NB300-130MFV500

mFluor Violet 610 SE

NB300-130MFV610

PE

NB300-130PE

PerCP

NB300-130PCP

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, C. elegans, Chicken, Drosophila, Equine, Monkey, Plant, Zebrafish

Applications

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ Clone # Ubi-1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Ubiquitin Antibodies »

Product Summary for Ubiquitin Antibody (Ubi-1) [CoraFluor™ 1]

Immunogen

Purified bovine erythrocyte ubiquitin cross-linked with glutaraldehyde to KLH

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 19147500).

Specificity

Recognizes polyubiquitin chains much more strongly than monoubiquitinated molecules or free ubiquitin. Specifically recognizes ubiquitinated cytoplasmic inclusion bodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Ubiquitin Antibody (Ubi-1) [CoraFluor™ 1]

Application

Recommended Usage

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Ubiquitin

Ubiquitin is a highly conserved protein of approximately 8.5kDa molecular weight, which has a normal role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C-terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

Alternate Names

UBB

Gene Symbol

RPS27A

Additional Ubiquitin Products

Product Documents for Ubiquitin Antibody (Ubi-1) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Ubiquitin Antibody (Ubi-1) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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