Skip to main content

StemXVivo Mesenchymal Stem Cell Expansion Media Best Seller

R&D Systems, part of Bio-Techne | Catalog # CCM004

R&D Systems, part of Bio-Techne

Key Product Details

Phenotypic Analysis of Human MSCs Expanded in StemXVivo® MSC Expansion Media. 
(3)

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, MSCs are cultured in expansion media using the following protocol:

  • Plate MSCs in expansion media
  • Culture MSCs to 80-90% confluency
  • Passage MSCs using fresh expansion media
 

 

Reagents Provided

Reagents supplied in the StemXVivo® Mesenchymal Stem Cell Expansion Media (Catalog # CCM004):

  • 250 mL of MSC expansion media

 

Other Supplies Required

Reagents

  • Penicillin-Streptomycin (100X)
  • Trypsin-EDTA (10X)
  • Phosphate-Buffered Saline (PBS)

Materials

  • MSCs (e.g., Rat Mesenchymal Stem Cells, Catalog # PSC003)
  • 75 cm2 tissue culture flasks
  • 15 mL centrifuge tubes
  • Serological pipettes
  • Pipettes and pipette tips

Equipment

  • 37 °C and 5% CO2 incubator
  • Centrifuge
  • Hemocytometer
  • Inverted microscope
  • 2 °C to 8 °C refrigerator
  • 37 °C water bath

 

Procedure Overview

This protocol has been tested using bone marrow- and/or adipose tissue-derived MSCs. If using a different tissue source or cell line, the protocol below may need to be modified.

Culturing Mesenchymal Stem Cells

Add 3.5 - 4.0 x 105 MSCs resuspended in 20 mL of the pre-warmed StemXVivo® MSC Expansion Media to a T75 flask.

Culturing Mesenchymal Stem Cells

Replace the medium every 3 days with fresh StemXVivo MSC Expansion Media.

Culturing Mesenchymal Stem Cells

Culture the MSCs to 80-90% confluency.

Subculturing Mesenchymal Stem Cells

Remove and discard the media from the T75 tissue culture flask(s) containing the MSCs.

Wash the cells with PBS.

Subculturing Mesenchymal Stem Cells

Add 1-2 mL of pre-warmed trypsin to the MSCs and incubate the flask at 37 °C.

Subculturing Mesenchymal Stem Cells

Transfer the dissociated MSCs to a 15 mL conical tube.

Centrifuge at 400 x g for 5 minutes.

Subculturing Mesenchymal Stem Cells

Resuspend the cell pellet in 5 mL of StemXVivo® MSC Expansion Media.

Subculturing Mesenchymal Stem Cells

Perform a cell count.

Subculturing Mesenchymal Stem Cells

Add 3.5-4.0 x 105 MSCs resuspended in 20 mL of pre-warmed StemXVivo® MSC Expansion Media to each T75 flask.

Subculturing Mesenchymal Stem Cells
Loading...
Loading...

FAQs for StemXVivo Mesenchymal Stem Cell Expansion Media

Loading...

Product Documents for StemXVivo Mesenchymal Stem Cell Expansion Media

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.