Human IL-10 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89871

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-89871
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum, plasma (citrate, heparin), buffered solution (50 uL)

Sensitivity

1.0 pg/mL

Assay Range

3.15 - 200 pg/mL

View all IL-10 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into a human IL-10 positive serum. There was no crossreactivity detected.

Description

The human IL-10 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IL-10.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 3.2

Inter-Assay Precision (Precision between assays) CV% < 5.6%

Recovery for Human IL-10 ELISA Kit (Colorimetric)

Recovery

The recovery ranged from 81% to 106% with an overall mean recovery of 97%. Please see protocol for more specific Spike Recovery details.

Scientific Data Images for Human IL-10 ELISA Kit (Colorimetric)

ELISA: Human IL-10 ELISA Kit (Colorimetric) [NBP1-89871]

ELISA: Human IL-10 ELISA Kit (Colorimetric) [NBP1-89871]

ELISA: IL-10 ELISA Kit [NBP1-89871] - Representative standard curve for human IL-10 ELISA. Human IL-10 was diluted in serial 2-fold steps in Assay Buffer (1x)

Kit Contents for Human IL-10 ELISA Kit (Colorimetric)

  • * reagent contains ethyl alcohol
  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IL-10
  • Amplification Diluent Concentrate (2x)
  • Amplification Reagent I*
  • Amplification Reagent II
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-human IL-10 monoclonal antibody
  • Control, lyophilized
  • Human IL-10 Standard lyophilized, 400 pg/mL upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-10

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non glycosylated polypeptide consisting of 178 amino acids. There is 73% homology between the human and mouse IL10 proteins, however, the human IL10 acts on both human and mouse target cells, while the mouse IL10 has species specific activity. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells and thymocytes. IL10 enhances cytotoxic T cell development, and costimulates B cell differentiation and immunoglobulin secretion. IL10 inhibits cytokine production by macrophages and suppresses macrophage class II MHC expression. The human IL10 gene is on human chromosome 1.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for Human IL-10 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human IL-10 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.