Rat IL-10 ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-92701

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Cell culture supernatant, serum (50 uL)

Sensitivity

1.5 pg/mL

Assay Range

15.6 - 1,000 pg/mL

View all IL-10 ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Rat

Specificity

The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a rat IL-10 positive serum. There was no crossreactivity detected.

Description

This kit is an enzyme-linked immunosorbent assay for quantitative detection.

Precision

Intra-Assay Precision (Precision within an assay) CV% < 5.0%

Inter-Assay Precision (Precision between assays) CV% < 10%

Scientific Data Images for Rat IL-10 ELISA Kit (Colorimetric)

ELISA: Rat IL-10 ELISA Kit (Colorimetric) [NBP1-92701]

ELISA: Rat IL-10 ELISA Kit (Colorimetric) [NBP1-92701]

ELISA: IL-10 ELISA Kit [Biotin] [NBP1-92701] - Standard curve

Kit Contents for Rat IL-10 ELISA Kit (Colorimetric)

  • Adhesive Films
  • Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to rat IL-10
  • Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  • Biotin-Conjugate anti-rat IL-10 monoclonal antibody
  • Rat IL-10 Standard lyophilized, 2 ng/ml upon reconstitution
  • Sample Diluent
  • Stop Solution (1M Phosphoric acid)
  • Streptavidin-HRP
  • Substrate Solution (tetramethyl-benzidine)
  • Wash Buffer Concentrate 20x (PBS with 1% Tween 20)

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-10

Interleukin-10 is a pleiotropic cytokine playing an important role as a regulator of lymphoid and myeloid cell function. Due to the ability of IL-10 to block cytokine synthesis and several accessory cell functions of macrophages, T-cells and NK cells. In addition, IL-10 participates in regulating proliferation and differentiation of B-cells, mast cells and thymocytes. The murine IL-10 exhibits strong DNA and aminoacid sequence homology to the human IL-10 and an open reading frame in the Epstein- Barr virus genome, BCRF1 which shares many of the cellular cytokine's biological activities and may therefore play a role in the host-virus interaction.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Gene Symbol

IL10

Additional IL-10 Products

Product Documents for Rat IL-10 ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat IL-10 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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