Mouse IL-27 ELISA Kit (Colorimetric)

Name: Mouse IL-27 ELISA Kit (Colorimetric)
Brand: Novus Biologicals
SKU: NBP3-06785

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06785

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Product Specifications
Precision
Recovery
Linearity
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

18.75 pg/mL

Assay Range

31.25 - 2000 pg/mL

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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Mouse

Specificity

This kit recognizes Mouse IL-27 in samples. No significant cross-reactivity or interference between Mouse IL-27 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.92

Inter-Assay Precision (Precision between assays) 5.21

Recovery for Mouse IL-27 ELISA Kit (Colorimetric)

Recovery

90 - 106 %

Linearity

Scientific Data Images for Mouse IL-27 ELISA Kit (Colorimetric)

ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] -

ELISA: Mouse IL-27 ELISA Kit (Colorimetric) [NBP3-06785] - Standard Curve Reference

Kit Contents for Mouse IL-27 ELISA Kit (Colorimetric)

Biotinylated Detection Ab Diluent
Concentrated Biotinylated Detection Ab (100x)
Concentrated HRP Conjugate (100x)
Concentrated Wash Buffer (25x)
HRP Conjugate Diluent
Micro ELISA Plate (Dismountable)
Plate Sealer
Product Manual
Reference Standard
Sample Diluent
Stop Solution
Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: IL-27

IL27 is encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR).

Long Name

Interleukin 27

Alternate Names

IL27

Gene Symbol