Recombinant Cynomolgus LIFR alpha His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10529-LR

R&D Systems, part of Bio-Techne
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10529-LR-050
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Key Product Details

Source

HEK293

Accession #

XP_005556818.1

Conjugate

Unconjugated

Applications

Bioactivity

View all LIFR alpha Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived cynomolgus monkey LIFR alpha protein
Gln45-Ser833, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln45 (blocked); deduced from Lys46 upon deblocking

Predicted Molecular Mass

90 kDa

SDS-PAGE

113-132 kDa, under reducing conditions

Activity

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is typically 0.7-7 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.

Scientific Data Images for Recombinant Cynomolgus LIFR alpha His-tag Protein, CF

Recombinant Cynomolgus LIFR alpha His-tag Protein Bioactivity

Recombinant Cynomolgus LIFR alpha His-tag Protein Bioactivity

Measured by its ability to inhibit LIF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.7-7 μg/mL in the presence of 0.3 ng/mL of recombinant human LIF.
Recombinant Cynomolgus LIFR alpha His-tag Protein SDS-PAGE

Recombinant Cynomolgus LIFR alpha His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Cynomolgus LIFR alpha His-tag Protein (10529-LR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 113-150 kDa.

Formulation, Preparation and Storage

10529-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: LIFR alpha

Leukemia Inhibitory Factor Receptor alpha (LIF R alpha), also known as LIFR beta and CD118, is a 190 kDa type I transmembrane protein in the Interleukin-6 receptor family. Members of this family mediate the biological effects of Cardiotrophin-1, CLC, CNTF, IL-6, IL-11, IL-27, and Oncostatin M (1). Based on its similarity with human LIF R alpha,  mature cynomolgus LIF R alpha is predicted to consist of a 789 amino acid (aa) extracellular domain (ECD) with two cytokine receptor homology domains, one WSxWS motif, and three fibronectin type III repeats, followed by a 26 aa transmembrane segment and a 238 aa cytoplasmic domain (2). Within the ECD, cynomolgus LIF R alpha shares 97% aa sequence identity with human LIF R alpha. LIF R alpha binds the pleiotropic cytokine LIF with low affinity (3). Binding affinity is increased by the ligand-induced association of LIF R alpha with the signal transducing subunit gp130 (4, 6). The LIF R alpha/gp130 receptor complex also transduces Oncostatin M signals, although LIF R alpha alone does not interact with Oncostatin M (4). gp130 associates with different ligand-specific receptors to form signaling receptor complexes for the other IL-6 family ligands (1). The CNTF receptor is a ternary complex that contains CNTF R alpha and gp130 as well as LIF R alpha (6, 7). LIF R alpha is widely expressed, and LIF induces the proliferation, differentiation, and activation of cells in many tissues (8, 9). In particular, LIF R alpha plays an important role in several aspects of early pregnancy such as blastocyst implantation in the uterus (10-12).

References

  1. Muller-Newen, G. (2003) Science STKE 2003:pe40.
  2. Gearing, D.P. et al. (1991) EMBO J. 10:2839.
  3. Layton, M.J. et al. (1992) Proc. Natl. Acad. Sci. 89:8616.
  4. Gearing, D.P. et al. (1992) Science 255:1434.
  5. Giese, B. et al. (2005) J. Cell Sci. 118:5129.
  6. Ip, N.Y. et al. (1992) Cell 69:1121.
  7. Davis, S. et al. (1993) Science 260:1805.
  8. Metcalf, D (2003) Stem Cells 21:5.
  9. Kubota, Y. et al. (2008) J. Clin. Invest. 118:2393.
  10. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319.
  11. Stewart, C.L. et al. (1992) Nature 359:76.
  12. Cheng, J.-G. et al. (2001) Proc. Natl. Acad. Sci. 98:8680.

Long Name

Leukemia Inhibitory Factor Receptor alpha

Alternate Names

CD118, LIF R alpha, LIF R beta, LIFR, LIFRa

Entrez Gene IDs

3977 (Human); 16880 (Mouse); 81680 (Rat); 102131906 (Cynomolgus Monkey)

Gene Symbol

LIFR

UniProt

XP_005556818.1

Additional LIFR alpha Products

Product Documents for Recombinant Cynomolgus LIFR alpha His-tag Protein, CF

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Product Specific Notices for Recombinant Cynomolgus LIFR alpha His-tag Protein, CF

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