Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 10307-IL
Key Product Details
Source
CHO
Accession #
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived cynomolgus monkey IL-27 protein
Cynomolgus Monkey EBI-3 (Arg36-Lys244) Accession # XP_005587614.1 |
GSGSSRGGSGSGGSGGGGSKL | Cynomolgus Monkey IL-27a |
6-His tag |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg36
Predicted Molecular Mass
50 kDa
SDS-PAGE
54-65 kDa, under reducing conditions
Activity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
The ED50 for this effect is 0.75-6.0 ng/mL.
The ED50 for this effect is 0.75-6.0 ng/mL.
Scientific Data Images for Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF
Recombinant Cynomolgus Monkey IL-27 His-tag Protein Bioactivity
Measured in an anti-viral assay using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.75-6.0 ng/mL.Recombinant Cynomolgus Monkey IL-27 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Cynomolgus Monkey IL-27 His-tag (Catalog # 10307-IL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 54-65 kDa.Formulation, Preparation and Storage
10307-IL
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with trehalose. |
Reconstitution | Reconstitute at 100 μg/mL in PBS. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IL-27
References
- Boulay, J-L. et al. (2003) Immunity 19:159.
- Trinchieri, G. et al. (2003) Immunity 19:641.
- Murakami, M. et al. (2004) Growth Factors 22:75.
- Cordoba-Rodriguez, R. and D.M. Frucht (2003) Exp. Opin. Biol. Ther. 3:715.
- Devergne, O. et al. (1996) J. Virology 70:1143.
- Pflanz, S. et al. (2002) Immunity 16:779.
- Villarino, A.V. et al. (2004) J. Immunol. 173:715.
- Pflanz, S. et al. (2004) J Immunol 172:2225.
- Scheller, J. et al. (2005) Biochem. Biophys. Res. Commun. 326:724.
- Holscher, C. (2004) Med. Microbiol. Immunol. 193:1.
Long Name
Interleukin 27
Alternate Names
IL27
Gene Symbol
IL27
Additional IL-27 Products
Product Documents for Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF
Product Specific Notices for Recombinant Cynomolgus Monkey IL-27 His-tag Protein, CF
For research use only
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