Recombinant Human Aldehyde Dehydrogenase 1-A1/ALDH1A1, CF
R&D Systems, part of Bio-Techne | Catalog # 5869-DH
Key Product Details
Source
E. coli
Accession #
Structure / Form
Noncovalently-linked homotetramer
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
E. coli-derived human Aldehyde Dehydrogenase 1-A1/ALDH1A1 protein
Ser2-Ser501, with an N-terminal Met and 6-His tag
Ser2-Ser501, with an N-terminal Met and 6-His tag
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser2
Predicted Molecular Mass
56 kDa
SDS-PAGE
57 kDa, reducing conditions
Activity
Measured by its ability to produce NADH during the oxidation of propionaldehyde.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.
The specific activity is >150 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
5869-DH
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, DTT and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aldehyde Dehydrogenase 1-A1/ALDH1A1
Aldehyde dehydrogenases (ALDHs) are NAD(P)+-dependent enzymes that detoxify aldehydes by oxidizing them to carboxylic acids. Nineteen ALDHs are present in humans, expressed in a variety of organelles and having different substrate preferences (1). ALDH1A1 is a cytosolic enzyme that preferentially oxidizes retinaldehyde to retinoic acid (2). ALDH1A1 is expressed in the epithelium of many organs, including brain, liver, testis, eye lens and cornea (3). ALDH1A1 is highly expressed in brain dopaminergic neurons, where it produces the retinoic acid required for their differentiation and development (4). The retinoic acid produced by ALDH1A1 is also important for the differentiation of hematopoietic stem cells (5). ALDH1A1 is a major enzyme in the oxidation of acetaldehyde, a toxic metabolite of ethanol (6).
References
- Zhao, D. et al. (1996) Eur. J. Biochem. 240:15.
- King, G. and Holmes, R. (1997) Adv. Exp. Med. Biol. 414:19.
- Jacobs, F.M. et al. (2007) Development 134:2673.
- Chute, J.P. et al. (2006) Proc. Natl. Acad. Sci. USA. 103:11707.
- Ueshima, Y. et al. (1993) Alcohol Alcohol. 1B:15.
Alternate Names
ALDC, Aldehyde Dehydrogenase 1A1, ALDH-E1, ALDH1A1, PUMB1, RALDH1
Entrez Gene IDs
216 (Human)
Gene Symbol
ALDH1A1
UniProt
Additional Aldehyde Dehydrogenase 1-A1/ALDH1A1 Products
- All Products for Aldehyde Dehydrogenase 1-A1/ALDH1A1
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 cDNA Clones
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 ELISA Kits
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Lysates
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Primary Antibodies
- Aldehyde Dehydrogenase 1-A1/ALDH1A1 Proteins and Enzymes
Product Documents for Recombinant Human Aldehyde Dehydrogenase 1-A1/ALDH1A1, CF
Product Specific Notices for Recombinant Human Aldehyde Dehydrogenase 1-A1/ALDH1A1, CF
For research use only
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