Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI2244

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P13688.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CEACAM-1/CD66a Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CEACAM-1/CD66a protein
Human CEACAM-1
(Gln35-Gly428)
Accession # P13688.2		 6-His tag Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln35

Predicted Molecular Mass

46 kDa

SDS-PAGE

85-105 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When 0.5 µg/mL (100 µL/well) of Biotinylated Recombinant Human CEACAM-1/CD66a His-tag Avi-tag (Catalog # AVI2244) is immobilized onto a Streptavidin coated plate (Catalog # CP004), Recombinant Human CEACAM-5/CD66e Fc Chimera  (Catalog # 10449-CM) binds with an ED50 of 50.0-500 ng/mL.

Scientific Data Images for Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human CEACAM‑1/CD66a His-tag Avi-tag Protein Binding Activity.

When 0.5 µg/mL (100 µL/well) of Biotinylated Recombinant Human CEACAM‑1/CD66a His-tag Avi-tag Protein (Catalog # AVI2244) is immobilized onto a Streptavidin coated plate (CP004), Recombinant Human CEACAM‑5/CD66e Fc Chimera (10449-CM) binds with an ED50 of 50.0-500 ng/mL

Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein (Catalog # AVI2244) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 85-105 kDa.

Formulation, Preparation and Storage

AVI2244
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CEACAM-1/CD66a

Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1), also known as cluster of differentiation 66a (CD66a) and biliary glycoprotein (BGP), is a member of the CEACAM subfamily of glycoproteins in the immunoglobulin (Ig) superfamily. The CEACAM family is involved in diverse cellular functions from cell adhesion and differentiation to proliferation, and survival and are utilized by several bacterial pathogens to bind and invade host cells (1-3). Mature human CEACAM-1 consists of an extracellular domain (ECD) with 1 V-type Ig-like domain and 3 C2-type Ig-like domains, a transmembrane domain, and a cytoplasmic region shows one ITIM motif and a calmodulin binding site (4). CEACAM-1 is unique among the CEACAM family as it is the only family member to contain the inhibitory ITIM domains. The ECD of human CEACAM-1 shares 54% and 52% amino acid sequence identity with mouse and rat CEACAM-1, respectively. Several alternative splice variants of CEACAM-1 have been reported with alterations occurring in both the ECD and cytoplasmic region, though the function of the isoforms remain poorly understood (5). CEACAM-1 can be expressed in human epithelial, endothelial, and hematopoietic cells and is involved in morphogenesis, apoptosis, angiogenesis, and cell proliferation as well as playing a role in innate and adaptive immunity (6,7). CEACAM-1 functions through either homophilic interactions with CEACAM-1 variants or binds in a heterophilic manner to other CEACAMs, including CEACAM-5, CEACAM-6, and CEACAM-8 (7). Several pathogens from Escherichia coli and Neisseria gonorrhoeae to N. meningitidis use CEACAM-1 as a ligand as a step in bacterial invasion of the host (8). CEACAM-1 signaling is associated with inhibition of proliferation and misregulation of its expression is often reported in cancer (5, 9, 10). As such, CEACAM-1 is being studied as a clinical biomarker and/or promising therapeutic target several cancer types including: non-small cell lung cancer, pancreatic, colorectal, bladder, and melanomas (5, 9, 10). Our Avi-tag Biotinylated Recombinant Human CEACAM‑1 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide.  Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Tchoupa, A. et al. (2014) J. Cell Commun. Signal 12:27.
  2. Hauck, C.R. et al. (2006) Eur. J. Cell Biol. 85:235
  3. Kuespert, K. et al. (2006) Curr Opin. Cell Biol. 18:565.
  4. Kim, W.M. et al. (2019) Seminars in immunology 42:101296.
  5. Dankner, M. et al. (2017) Oncoimmunology. 6:e1328336.
  6. Gandhi, A.K. et al. (2021) Commun Biol 4:360.
  7. Helfrich, I. and Singer, B.B. (2019) Cancers 11:356.
  8. Voges, M. et al. (2010) BMC Microbiol. 10:117.
  9. Beauchemin, N. and Arabzadeh, A. (2013) Cancer Metastasis Rev. 32:643.
  10. Fiori, V. et al. (2012) Ann Ist Super Sanita. 48:161.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 1

Alternate Names

BGP1, Biliary Glycoprotein 1, CD66a, Cea-1, CEACAM1, Hv-1, Hv-2, MHVR

Entrez Gene IDs

634 (Human); 26365 (Mouse); 102139857 (Cynomolgus Monkey)

Gene Symbol

CEACAM1

UniProt

P13688.2

Additional CEACAM-1/CD66a Products

Product Documents for Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CEACAM-1/CD66a His-tag Avi-tag Protein, CF

For research use only

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