Recombinant Human IL-2 Heat Stable Agonist Protein, CF
R&D Systems, part of Bio-Techne | Catalog # BT-002HS
AI Modified Protein
Engineered using artificial intelligence (AI), this protein has enhanced stability, enabling it to withstand high temperatures and extended culture durations for your T cells and tumor-infiltrating lymphocytes. Learn more in this Application Note.
Key Product Details
- Simple to Use- Optimized for T Cells & Tumor Infiltrating Lymphocytes-IL-2 Heat Stable Agonist was modeled to engage only the IL-2 dimeric ßγ receptor complex
- Improved Stability- Enhanced stability facilitated by a synthetic core engineered to strengthen the protein's structure, enabling it to withstand high temperatures and extended culture durations.
- Superior Capabilities- Higher bioactivity at 37 oC compared to standard IL-2.
Source
E. coli
Accession #
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived human IL-2 protein
Proprietary
Proprietary
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
SDS-PAGE
7 kDa, under reducing conditions.
Activity
Measured in a cell proliferation assay using NK-92 human natural killer lymphoma cells.
The ED50 for this effect is 0.0150-0.150 ng/mL.
The ED50 for this effect is 0.0150-0.150 ng/mL.
Scientific Data Images for Recombinant Human IL-2 Heat Stable Agonist Protein, CF
Heat Stable IL-2 retains activity for 59 days at 37 °C
Proliferation bioassay in NK92 cells after 59 days in media at 37 °C. Higher stability allows for less dosing throughout extended T cell cultures as well as early media prep to minimize culture inconsistencies from degrading cytokines.
Proliferation bioassay in NK92 cells after 10 min at 95 °C
After 10 min at 95°C (Control IL-2 C145S was kept at 4°C), only the heat stable IL-2 (Cat # BT-002HS) maintained bioactivity similar to the control IL-2, whereas the IL-2 Aldesleukin protein lost >14-fold activity.
Proliferation bioassay in NK92 cells after 10 days at 37°C
After 10 days at 10 ng/mL in the cell culture medium at 37°C (Control IL-2 C145S was kept at 4°C), only the heat stable IL-2 (Cat # BT-002HS) maintained bioactivity similar to the control IL-2, whereas the IL-2 Aldesleukin protein lost >3-fold activity.
Formulation, Preparation and Storage
BT-002HS
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Reconstitution | Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: IL-2
Predictive analytics are increasingly effective in solving challenges associated with developing protein reagents for cell culture applications, including thermal and pH instability. We have improved the stability of IL-2 by generating a synthetic structural core while maintaining efficiency in engagement and activation of IL-2 R beta/ gammac receptors. The enhanced IL-2 displays improved heat stability enabling it to retain bioactivity while withstanding elevated temperatures and extended culture durations. Recombinant IL-2 is a bioactive protein for use
in cell culture applications, often used for the expansion of T cells. IL-2 is
expressed by CD4+ and CD8+ T cells, gamma delta T cells, B cells, dendritic
cells, and eosinophils (1-3). rhIL-2 is also used for the expansion of NK
cells, early differentiated T cells and effector memory Treg cells for adoptive
cell transfer cancer immunotherapy (4,5). rhIL-2 Heat Stable agonist is
verified for activity using an NK92 proliferation assay as well as T Cell
culture applications comparing to the commonly used mutant aldesleukin IL-2
version.
References
- Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
- Gaffen, S.L. and K.D. Liu (2004) Cytokine 28:109.
- Taniguchi, T. et al. (1983) Nature 302:305.
- Koehl, U. et al. (2015) Oncoimmunology. 5:e1115178.
- Chamucero-Millares, J.A. et al. (2021) Cellular Immunol. 360:104257.
Long Name
Interleukin 2
Alternate Names
Aldesleukin, IL2, Proleukin, TCGF
Entrez Gene IDs
Gene Symbol
IL2
UniProt
Additional IL-2 Products
Product Documents for Recombinant Human IL-2 Heat Stable Agonist Protein, CF
Product Specific Notices for Recombinant Human IL-2 Heat Stable Agonist Protein, CF
For research use only
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