Recombinant Human MMP-12 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 917-MPB

R&D Systems, part of Bio-Techne
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917-MPB-020
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Key Product Details

Source

NS0

Accession #

P39900

Structure / Form

Pro form

Conjugate

Unconjugated

Applications

Enzyme Activity

View all MMP-12 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human MMP-12 protein
Leu17-Cys470, with Val97Leu substitution

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu17

Predicted Molecular Mass

52 kDa

SDS-PAGE

53-60 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The specific activity is >500 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

917-MPB
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, CaCl2, CHAPS, ZnSO4 and PEG.
Reconstitution Reconstitute at 0.25 mg/mL in 10 mM MES, 0.1 M NaCl, 100 μM CaCl2, 0.1% CHAPS, 1 μM ZnSO4 and 0.1% PEG., pH 6.0.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMP-12

Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases that collectively degrade the components of the extracellular matrix. MMP-12 (macrophage elastase) consists of the following domains: a pro domain, a catalytic domain containing the zinc-binding site, and a C-terminal hemopexin-like domain. The 54 kDa rhMMP-12 pro form is activated via processing into 45 kDa and 22 kDa active forms (1). MMP-12 is capable of cleaving several substrates in addition to elastin. Macrophage secretion of MMP-12 at sites of inflammation can be induced by cytokines. Given the secretion of MMP12 during an inflammatory response, MMP-12 is involved in many pathologies including vascular disease (2, 3) and cancer (4-6). In particular, MMP12-mediated pathological degradation of the extracellular matrix is a well-established key event in inflammatory-related pulmonary disease (7). For example, overexpression of MMP12 in alveolar macrophages is associated with smoking and emphysema (8) while different MMP-12 variants result in protection against chronic obstructive pulmonary disease (COPD) development (9) or cause increased risk and disease severity (10). MMP-12 has also been shown to translocate into the nucleus of viral-infected cells to directly regulate transcription during an immune response (11).

References

  1. Shapiro, S.D. et al. (1993) J. Biol. Chem. 268:23824.
  2. Liu, S.L. et al. (2015) Sci. Rep. 5:17189.
  3. Iyer, R.P. et al. (2015) Int. J. Cardiol. 185:198.
  4. Cao, W. et al. (2017) Oncol. Rep. 3:1401.
  5. Klupp, F. et al. (2016) BMC Cancer 16:494.
  6. Chung, I.C. et al. (2014) BMC Cancer 14:348.
  7. Vanderbroucke, R.E. et al. (2011) Eur. Respir. J. 387:1200.
  8. Babusyte, A. et al. (2007) Respir. Res. 8:81.
  9. Hunninghake, G.M. et al. (2009) N. Engl. J. Med. 361:2599.
  10. Mukhopadhyay, S. et al. (2010) J. Allergy Clin. Immunol. 126:70.
  11. Marchant, D.J. et al. (2013) Nature Medicine 20:493.

Long Name

Matrix Metalloproteinase 12

Alternate Names

MMP12

Entrez Gene IDs

4321 (Human); 17381 (Mouse)

Gene Symbol

MMP12

UniProt

P39900

Additional MMP-12 Products

Product Documents for Recombinant Human MMP-12 Protein, CF

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Product Specific Notices for Recombinant Human MMP-12 Protein, CF

For research use only

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