Recombinant Human TIMP-2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 971-TM
Key Product Details
Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human TIMP-2 protein
Cys27-Pro220
Cys27-Pro220
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Cys27
Predicted Molecular Mass
21 kDa
SDS-PAGE
22-23 kDa, reducing conditions
Activity
Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (Catalog # ES001).
The IC50 value is approximately 2.2 nM, as measured under the described conditions.
The IC50 value is approximately 2.2 nM, as measured under the described conditions.
Reviewed Applications
Read 2 reviews rated 4.5 using 971-TM in the following applications:
Formulation, Preparation and Storage
971-TM
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, and 0.05% Brij-35, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: TIMP-2
Tissue inhibitors of metalloproteinases or TIMPs are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4. TIMP-2 is a non N-glycosylated protein with a molecular mass of 22 kDa produced by a wide range of cell types, which inhibits MMPs non-covalently by the formation of binary complexes. TIMP-2 also has erythroid‑potentiating and cell growth promoting activities.
Long Name
Tissue Inhibitors of Metalloproteinases 2
Alternate Names
TIMP2
Gene Symbol
TIMP2
UniProt
Additional TIMP-2 Products
Product Documents for Recombinant Human TIMP-2 Protein, CF
Product Specific Notices for Recombinant Human TIMP-2 Protein, CF
For research use only
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