Recombinant Mouse CLEC-2/CLEC1B Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9117-CL

R&D Systems, part of Bio-Techne
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9117-CL-050
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Key Product Details

Source

NS0

Accession #

Q9JL99

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC-2/CLEC1B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CLEC-2/CLEC1B protein
Met53-Leu229, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

21 kDa

SDS-PAGE

24-39 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 1.0 µg/mL (100 μL/well), Recombinant Mouse CLEC-2/CLEC1B binds with a typical ED50 of 0.1-0.5 μg/mL.

Scientific Data Images for Recombinant Mouse CLEC-2/CLEC1B Protein, CF

Recombinant Mouse CLEC-2/CLEC1B Protein Bioactivity

Recombinant Mouse CLEC-2/CLEC1B Protein Bioactivity

When Recombinant Mouse Podoplanin Fc Chimera (Catalog # 3244-PL) is coated at 1.0 µg/mL, Recombinant Mouse CLEC-2/CLEC1B (Catalog # 9117-CL) binds with a typical ED50 of 0.1-0.5 µg/mL.

Formulation, Preparation and Storage

9117-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC-2/CLEC1B

C-type lectin-like receptor 2, also known as CLEC-1B, is a 32 kDa type II transmembrane glycoprotein that is expressed on platelets, megakaryocyte, and liver sinusoidal cells (1-4). Mature mouse CLEC-2 consists of a 31 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 177 aa extracellular domain (ECD) with one C-type lectin domain. Within the ECD, mouse CLEC-2 shares 59% and 90% aa sequence identity with human and rat CLEC-2, respectively. Alternative splicing generates a short isoform that lacks the transmembrane segment. CLEC-2 binding to Podoplanin trigger platelet activation, shedding of GPVI, and cleavage of Fcg RIIA/CD32a (5-8). CLEC-2 expression on platelets is required for the development of lymphatic vessels and valves as well as the maintenance of lymph node high endothelial veinules (HEV) during lymphocyte crossing (7-10). CLEC-2 additionally binds to fucoidan, HIV-1, and the platelet-aggregating snake venom protein Rhodocytin/Aggretin (2, 3, 11). In the liver, soluble CLEC-2 promotes M2 macrophage differentiation and limits inflammatory cytokine production (12).

References

  1. Lee, R.H. and W. Bergmeier (2016) J. Thromb. Haemost. Epub PMID 26749528.
  2. Suzuki-Inoue, K. et al. (2006) Blood 107:542.
  3. Chaipan, C. et al. (2006) J. Virol. 80:8951.
  4. Gitz, E. et al. (2014) Blood 124:2262.
  5. Suzuki-Inoue, K. et al. (2007) J. Biol. Chem. 282:25993.
  6. Christou, C.M. et al. (2008) Biochem. J. 411:133.
  7. Herzog, B.H. et al. (2013) Nature 502:105.
  8. Sweet, D.T. et al. (2015) J. Clin. Invest. 125:2995.
  9. Hess, P.R. et al. (2014) J. Clin. Invest. 124:273.
  10. Osada, M. et al. (2012) J. Biol. Chem. 287:22241.
  11. Manne, B.K. et al. (2013) J. Biol. Chem. 288:7717.
  12. Wu, X. et al. (2015) EbioMedicine 2:214.

Long Name

C-type Lectin-like Receptor 2

Alternate Names

CLEC1B, CLEC2

Entrez Gene IDs

51266 (Human); 56760 (Mouse)

Gene Symbol

CLEC1B

UniProt

Q9JL99

Additional CLEC-2/CLEC1B Products

Product Documents for Recombinant Mouse CLEC-2/CLEC1B Protein, CF

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