Key Product Details

Species Reactivity

Mouse

Applications

Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all IgG2a Heavy Chain Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG2a heavy chain

Specificity

Minimal cross reactivity was noted against other Mouse immunoglobulin classes or subclasses.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum

Scientific Data Images for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 488]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 488] [NBP1-72891] - This image shows anti-histone detection using a DyLight (TM) 488 conjugate (green). Anti-Tubulin was detected using a DyLight (TM) 549 conjugate (red). Nuclei were counter-stained using DAPI (blue). The image was captured using an Axio Imager.Z1.
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 488] [NBP1-72891] - Comparison of fluorescence adsorption and emission spectra for Dylight 488 and Alexa Fluor 488 in PBS, pH 7.2.

Applications for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 488]

Application
Recommended Usage

Fluorophore-linked immunosorbent assay

1:20000

Immunocytochemistry/ Immunofluorescence

1:5000

Western Blot

1:10000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight(TM) conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 488]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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