Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [Allophycocyanin/Cy7]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-72794APCCY7
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Dot Blot, ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunohistochemistry, Western Blot
Label
Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Mouse IgG2a heavy chain
Specificity
Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a Heavy Chain, IgG).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Applications
Application
Recommended Usage
Dot Blot
Optimal dilutions of this antibody should be experimentally determined.
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Fluorophore-linked immunosorbent assay
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.
Formulation, Preparation, and Storage
Purification
Multi-step
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: IgG2a Heavy Chain
Additional IgG2a Heavy Chain Products
Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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