Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [HRP]

Mouse

Dot Blot, ELISA, Immunohistochemistry, Western Blot

HRP

Polyclonal Rabbit IgG

LYOPH mg/ml

Mouse IgG2a heavy chain

Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or heavy or light chain isotypes.

Polyclonal

Rabbit

IgG

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Mouse IgG and Mouse Serum

Multi-step

Reconstitute with 1.0 ml deionized water (or equivalent).

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

0.01% Gentamicin Sulfate

LYOPH mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.