Key Product Details

Species Reactivity

Mouse

Applications

Dot Blot, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

LYOPH mg/ml

View all IgG2a Heavy Chain Secondary Antibodies »

Product Summary for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

Immunogen

Mouse IgG2a heavy chain

Specificity

Specificity was confirmed by ELISA.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of immobilized mouse and human serum proteins resulting in a conjugated antibody designed for flow cytometry

Scientific Data Images for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC] [NBP1-73637] - Western blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [FITC].Lane 1: Mouse IgG2a.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody [FITC] at 1:1,000 for 60 min at RT.Blocking buffer for 30 min at RT.Predicted/Observed size: 55 kDa, 55 kDa for Mouse IgG2a (Gamma 2a chain).Other band(s): None.
Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC] [NBP1-73637]
Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

Dot Blot results of Rabbit Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]. Dots are Mouse IgG2a at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. blocking for 30 min at RT. Primary Antibody: Rabbit Anti-Mouse IgG2a FITC at 1ug/mL for 1hr at RT. Secondary Antibody: none. Imaged with BioRad ChemiDoc, FITC filter.
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Applications for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

Application
Recommended Usage

Flow Cytometry

1:500 - 1:2500

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000
Application Notes
This product has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Product Documents for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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