para-Nitroblebbistatin

Name: para -Nitroblebbistatin
Brand: Tocris Bioscience
SKU: 8804

Tocris Bioscience, part of Bio-Techne | Catalog # 8804

COA / SDS

Selective inhibitor of myosin II ATPase activity. Non-cytotoxic version of (±)-Blebbistatin (Cat. No. 1760)

Key Product Details

Description:	Selective inhibitor of myosin II ATPase activity. Non-cytotoxic version of (±)-Blebbistatin (Cat. No. 1760)
Chemical Name:	(3aS)-1,2,3,3a-Tetrahydro-3a-hydroxy-6-methyl-1-(4-nitrophenyl)-4H-pyrrolo[2,3-b]quinolin-4-one
Purity:	≥98% (HPLC)
Molecular Weight:	337.34

View Full Technical Details for para-Nitroblebbistatin

Technical Data
Citations

Biological Activity for para-Nitroblebbistatin

para-Nitroblebbistatin is a selective inhibitor of Myosin II ATPase activity (IC₅₀ = 15 μM). It is a non-fluorescent, photostable, non-cytotoxic and non-phototoxic derivative of (±)-Blebbistatin (Cat. No. 1760).

(S)-(-)-Blebbistatin (Cat. No. 1852) also available.

Technical Data for para-Nitroblebbistatin

M. Wt	337.34
Formula	C₁₈H₁₅N₃O₄
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	1621326-32-6
PubChem ID	102361739
InChi Key	KAUXNLHXGQGFOS-GOSISDBHSA-N
Smiles	O[C@@]12C(N(C3=CC=C(C=C3)[N+]([O-])=O)CC2)=NC4=CC=C(C)C=C4C1=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for para-Nitroblebbistatin

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	16.87	50

Preparing Stock Solutions for para-Nitroblebbistatin

The following data is based on the product molecular weight 337.34.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.5 mM	5.93 mL	29.64 mL	59.29 mL
2.5 mM	1.19 mL	5.93 mL	11.86 mL
5 mM	0.59 mL	2.96 mL	5.93 mL
25 mM	0.12 mL	0.59 mL	1.19 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for para-Nitroblebbistatin

References are publications that support the biological activity of the product.

Képiró para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor Angew.Chem Int.Ed.Engl. 2011 PMID: 24954740
Värkuti A highly soluble, non-phototoxic, non-fluorescent blebbistatin derivative. Sci.Rep. 2016 PMID: 27241904
Gollapudi Two classes of myosin inhibitors, para-nitroblebbistatin and mavacamten, stabilize β-cardiac myosin in different structural and functional states. J.Mol.Biol. 2021 PMID: 34627791