RNAscope™ HiPlex Pro was developed by ACD and Lunaphore to detect up to 12 RNA targets in a method that is optimized for RNA and protein co-detection based on seqIF™ (sequential immunofluorescence) on Lunaphore's hyperplex platform, COMET™. Combining both assays RNAscope HiPlex Pro with seqIF on COMET provides provides end-users with a fully-automated, same-section assay integrating multiplex RNA and multiplex protein data without compromising panel flexibility or sensitivity. The combined workflow allows users to select any 12 RNAscope targets and up to 24 protein targets using off-the-shelf, non-conjugated primary antibodies. The assay is designed to be compatible with current procedures on COMET using a unique protease-free pretreatment to promote streamlined, spatial multiomic data acquisition with subcellular resolution.
Same-Section Multiomics
Same-Section Multiomics
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Single workflow for co-detection of RNA and protein targets on the same tissue section
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Generate spatially resolved multiomic data at subcellular resolution
Fully-Automated Workflow
Fully-Automated Workflow
- Automated from target probe hybridization and protein detection to imaging
- Stain up to 4 slides in every run
Flexible Assay Design
Flexible Assay Design
- Use our extensive RNAscope probe catalog or design custom probes for RNAs
- Design custom panels
Technology Overview
Fully-Automated, Protease-Free Workflow: Automated probe hybridization, signal amplification, labeling, imaging and cleaving follow the multiomic-optimized deparaffinization and antigen retrieval steps. Each round allows the detection of 4 RNA targets for up to 3 rounds which can be followed by seqIF protocols.
Featured Applications
Immunology
Immunology
- Assess cytokine profiles in inflamed tissues and identify the cellular source of inflammatory molecules
- Visualize gene signatures with cell-specific markers to assess activation states of immune cells
Immuno-oncology
Immuno-oncology
- Evaluate the tumor immune context for a better therapeutic outcome
- Study tumor heterogeneity by visualizing unique morphology and expression profiles
Immunity and Infectious Disease
Immunity and Infectious Disease
- Localization—visualizing infected cell types and tissues enables elucidation of viral transmission, pathogen-host interactions, and localization of hidden reservoirs
- Differential detection—targeting the sense or anti-sense strand enables detection and differentiation of virus during latent or active stages
- Coinfection detection—detect multiple viruses or co-infection
Cytokine Detection with Multiomics on COMET
Sample Type: FFPE human breast cancer
RNA: IL6 (green)
Protein: CD3 (yellow), Vimentin (orange), aSMA (blue)
Search Antibodies
Work with off-the-shelf, non-conjugated primary antibodies or quickly start using SPYRE Antibody Panels and detection reagents from Lunaphore.
Get Started Today. Speak With A Specialist.
Watch our webinar on profiling the tumor microenvironment through same-section fully automated spatial multiomics.
| Type | Component | Catalog # |
|---|---|---|
| Reagent Kits | RNAscope HiPlex Pro for COMET 4-plex, 20-slide Reagent Kit | 322085 |
| RNAscope HiPlex Pro for COMET 12-plex, 20-slide Reagent Kit | 322075 | |
| Negative Control Probe | RNAscope HiPlex12 CS Negative Control Probe | 324347 |
| Positive Control Probes | RNAscope HiPlex12 CS Positive Control Probe-Hs | 324317 |
| RNAscope HiPlex12 CS Positive Control Probe-Mm | 324437 | |
| RNAscope HiPlex12 CS Positive Control Probe-Rn | 324337 |
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