Target Validation and Targeted Protein Degradation
Target validation is the process of establishing that a potential molecular target is involved in a physiological function or disease mechanism. Target identification and validation are important steps in drug discovery and biological research. Target validation can be achieved by modulation of a potential target and exploring the downstream molecular and/or phenotypic consequences. Protein Degraders and the field of Targeted Protein Degradation offer a differentiated way to modulate target proteins using small molecules. While a small molecule inhibitor will block or modulate a specific protein domain or function (for example an enzymatic role), a small molecule Degrader will knockdown the entire protein, removing all possible functions. Target validation is therefore an important stage of the Degrader development project workflow. The validation of a protein target then supports the design and chemical synthesis of a Degrader or other small molecules selective for that target.
Bio-Techne has a range of tools available for target identification and validation including our TAG Degradation Platform and Protein Degraders.
Protein Degraders
Bio-Techne provides a large catalog of off-the-shelf Protein Degraders (including: PROteolysis TArgeting Chimeras or PROTAC® molecules; SNIPERs; PHOTACs and uSMITE™ molecules) for a wide range of target proteins. Targeted protein degradation does not always phenocopy inhibition and Protein Degraders often display an enhanced selectivity profile compared to the warhead inhibitor used. Our Protein Degraders are therefore a highly useful tool to explore and validate target proteins and can be used to investigate the phenotypic consequences of proteasome-mediated protein knock-down.
TAG Degradation Platform for Target Validation
TAG Degradation technology offers a generalizable strategy to degrade, in principle, any intracellular protein of interest. This target validation methodology requires a protein of interest to be expressed as a fusion protein with a TAG domain, also known as a degron tag, either via transgene expression or CRISPR-mediated locus-specific knock-in. The small molecule TAG Degrader (e.g. dTAG, aTAG or BromoTag®) recruits the fusion protein for ubiquitination by an E3 ligase, which targets it for destruction by the proteasome.
For more information on how TAG Degradation works, check out the dTAG/aTAG/BromoTag Degraders page.
Bio-Techne offers a full range of products and services across the TAG Degradation Platform Workflow including dTAG, aTAG and BromoTag Degraders, as well as Simple Western™ instruments and validated antibodies for western blotting to measure target protein knockdown.
Products and Services for the TAG Degradation Platform
dTAG, aTAG and BromoTag Degraders
dTAG, aTAG and BromoTag Degraders
Our dTAG, aTAG and BromoTag Degrader molecules target fusion proteins comprising your target of interest fused to a protein tag. This technology allows rapid and highly selective degradation of a protein of interest and offers a useful alternative to genetic methods for target validation. It can be used in cell culture or in vivo.
Simple Western
Simple Western
Detect Degrader-induced knock-down of your target protein of interest with Simple Western. Our Simple Western™ platforms automate the entire protein separation and detection process, enabling you to accurately measure DC50 and Dmax.
Resources for Target Validation and Exploration
Targeted Protein Degradation Western Blot Solutions
Targeted Protein Degradation Western Blot Solutions
Our completely automated Simple Western™ platform supports high throughput protein degradation workflows to provide reproducible and quantitative DC50 data. The systems integrate into PROTAC® Degrader and Molecular Glue Degrader screening assays and have a growing range of antibodies validated for use in both Western Blot and Simple Western™ instruments.
Validated Antibodies for Simple Western™ and Western Blot
Validated Antibodies for Simple Western™ and Western Blot
Bio-Techne supply a range of antibodies validated for Western blot and for our Simple Western™ gel-free, blot-free and hands-free Western Blot instruments. We are constantly developing new antibodies to help you evaluate the efficacy of your Degrader.
Bio-Techne's Complete Targeted Protein Degradation Workflow Solution
Bio-Techne's Complete Targeted Protein Degradation Workflow Solution
Bio-Techne offers a unique portfolio of high-quality reagents, instruments, and services for your protein degradation pathway research. Our Research Product Guide highlights our tools and services to support your Targeted Protein Degradation and Induced Proximity research.
Whitepaper: Validating Targets for TPD using dTAG
Whitepaper: Validating Targets for TPD using dTAG
Learn about our comprehensive workflow solution for target validation in targeted protein degradation using the TAG Degradation Platform. The whitepaper provides guidance on TAG knock-in cell-line generation, using dTAG Degraders to knockdown your protein of interest, and assays for protein degradation using Simple WesternTM instruments and western blot-validated antibodies.
Research Paper: Development of an AchillesTAG Degradation System
Research Paper: Development of an AchillesTAG Degradation System
Our paper with C4 Therapeutics highlights the use of the AchillesTag (aTAG) targeted protein degradation technology to control Chimeric Antigen Receptor (CAR-T) activity.
PROTAC® is a registered trademark of Arvinas Operations, Inc., and is used under license.
BromoTag® is a registered trademark of the University of Dundee and is used under license