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Being able to spatially resolve cells in tissue using tools like RNAscope™ …will have a lot of clinical relevance in the future… that’s where the real power of this can be seen going from bench to bedside.
The RNAscope assay was very helpful in discovering this, not only because we were able to confidently discern between the different sodium channels, but also because we could use the RNAscope Multiplex Fluorescent kit v2 in situ assay to co-localize gene expression with cell types.
With its importance in understanding causality and the viral-host relationship, RNAscope technology has been a vital tool in my research... a method as sensitive as RNAscope assay with spatial resolution is absolutely irreplaceable for our confidence in determining viral contribution to a disease.
In fact, we would certainly recommend RNAscope technology to researchers wanting to examine a target of interest for which there is not a reliable antibody available
Because RNAscope can detect expression of multiple genes in parallel, it can tell us whether those genes discovered through RT-PCR are co-expressed in the same cell or not – information that is very valuable and hard to obtain by other means.
The development of combined protein and RNA detection methods may alleviate many concerns for accurate detection of biomarkers, and I can see this being the standard practice in future molecular cancer profiling... Given the unique probe design and detection chemistry of the RNAscope method, we were able to get results with high specificity and sensitivity for many probes that we have used in my laboratory. To date we don’t have any comparable technology for the reliable detection of RNA in FFPE tissues other than RNAscope.
The routine detection of non-coding RNA presents a particular set of challenges since they do not have protein counterparts for antibody detection. This is why the ability to detect RNA in cancer biopsy samples is so valuable, opening up the use of lncRNA as a biomarker.
Using the RNAscope assay probe against LGR5 mRNA has allowed us to study the localization of LGR5 expression without relying on an antibody. I would certainly recommend RNAscope technology to researchers wanting to examine a target of interest for which there is not a reliable antibody available.
Simple Western
Jess is faster and more reliable in terms of quantification and allows us to use low sample volumes, for example, difficult-to-obtain samples such as iPSC-derived neurons.
I've enjoyed teaching and mentoring more than I ever expected, and knowing that about myself has allowed me to pursue a specific career track as I apply for faculty positions that strongly emphasize undergraduate education.
Don't disregard results because an experiment did not turn out the way you or your PI expected.
I think this is something all immunologists can agree on - trusted sources of research tools/reagents can be the difference between a high impact publication and no publication/delayed publication.
