Why Should You Graduate From SDS-Page?
Sodium dodecyl sulfate – polyacrylamide gel electrophoresis, popularly known as SDS-PAGE, is widely used for the separation of proteins based on molecular weight. If you use SDS-PAGE regularly, you know its limitations:
- Time-consuming manual process
- Qualitative information
- Error-prone, resulting in data variability
- Low reproducibility that hinders publication
Did you know that SDS-PAGE has a modern version, CE-SDS, that addresses all the limitations mentioned above?
What are the Advantages of CE-SDS over SDS-PAGE?
Short for capillary electrophoresis-sodium dodecyl sulfate, CE-SDS functions on a similar principle to SDS-PAGE, but simplifies your workflow and offers the following advantages:
- Reduces the potential for human error through automation
- Accelerates your research through fast and quantitative results, in as little as 5 minutes
- Boosts productivity with high throughput
- Makes you confident in your findings with reproducible data
- Assures compliance with regulatory standards
Who Uses CE-SDS?
Did you know that CE-SDS is recognized by regulatory authorities as one of the standard methods in the biopharmaceutical industry? In fact, the United States Pharmacopeia (USP) General Chapter <129> provides specific guidelines for the analysis of therapeutic monoclonal antibodies using CE-SDS and size-exclusion chromatography.
Maurice CE-SDS Generates Comparable Data with USP <129>
Maurice CE-SDS Generates Comparable Data with USP <129>
Discover how the Maurice CE-SDS PLUS technique aligns with the analytical protocols outlined in USP General Chapter <129> for evaluating monoclonal antibodies.
A Key Opinion Leader Discusses Capillary Electrophoresis
A Key Opinion Leader Discusses Capillary Electrophoresis
Explore Prof. Dr. Hermann Wätzig's insights on capillary electrophoresis's evolution in biologics quality control, and its diverse applications in pharmaceutical analytics.
Time to Turn the PAGE on SDS-PAGE
Prof. Hermann Watzig and Dr. Chris Heger explore how CE-SDS is swiftly replacing SDS-PAGE worldwide, offering superior separation, quantitation, and automation capabilities.
How does CE-SDS with Maurice™ Work?
The Maurice™ system enables CE-SDS analysis, providing results in as little as 5.5 minutes per sample.
- Self-contained pre-assembled cartridges
- Automatic clean-up
- No messy gels or stains
- No need to set up any electrophoresis apparatus
Use in any lab
Use in any lab
- Turbo CE-SDS™ for discovery and high throughput
- CE-SDS PLUS for analytical development and QC
Choose your data format
Choose your data format
High-quality data in the form of lane view and/or electropherograms
Use in different applications
Use in different applications
Suitable for the analysis of mAbs, biosimilars, viral vectors, GMP cytokines, milk proteins, and more
Stay 21 CFR Part 11 compliant
Stay 21 CFR Part 11 compliant
Data can be analyzed with 21 CFR Part 11 compliant software including Compass for iCE, Waters™ Empower®, and Chromeleon™ CDS
| Aspect | SDS-PAGE | CE-SDS |
|---|---|---|
| Automation | Manual | Highly automated |
| Separation Medium | Polyacrylamide gel | Polymer-based sieving matrix |
| Reproducibility | Variable | High |
| Sample Loading | Manual | Automated/electrokinetic |
| Run Time | Hours | Minutes to an hour |
| Throughput | Lower | Higher |
| Consistency | Dependent on manual steps | Consistent due to pre-assembled cartridges |
| Quantification | Semi-quantitative | Quantitative |
| Imaging/Data Interpretation | Rsieving matriequires staining and destaining with potential for variability in image capture | Real-time UV absorbance provides immediate, reproducible results |
From Sodium Dodecyl Sulfate –Polyacrylamide Gel Electrophoresis to CE-SDS: Transforming Protein Analysis
Adopting a new analytical technique always raises some important questions. Take advantage of our resources below to get your pressing questions about CE-SDS vs SDS-PAGE answered!
Comparing SDS-PAGE with Maurice CE-SDS
Read our Application Note, on the comparison of Maurice™ CE-SDS with SDS PAGE in parallel, using a standard and a commercially available therapeutic monoclonal antibody (mAb) under varying conditions. The comparative results highlight the advantages of Maurice CE-SDS over SDS PAGE for routine product purity characterization.
Advantages of CE-SDS
Listen to DDW's podcast with Dr. Tufan Aydogdu, a Field Applications Scientist at ProteinSimple, a Bio-Techne brand, as they discuss the benefits of automated CE-SDS over SDS-PAGE.
Apparent Molecular Weight on CE-SDS vs SDS-PAGE
Read our technical note on relevant peer-reviewed publications addressing factors for comparing molecular weights between CE-SDS and SDS-PAGE.