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Human IgG Fc Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB110

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Western Blot

Cited:

Control, ELISA Development, Neutralization, Neutralization Control, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 97924

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IgG1 Fc region

Specificity

Detects chimeric proteins containing a human IgG Fc region in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IgG Fc Antibody

Detection of IgG1 Fc antibody in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP antibody by Flow Cytometry.

Detection of IgG1 Fc in Human B7-H1/PD-L1 Fc Chimera Bound to HEK293 Human Cell Line Transfected with Human PD-1 and eGFP by Flow Cytometry.

(A) Recombinant Human B7-H1/PD-L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). (B) In the absence of rhB7-H1/PD-L1 Fc Chimera, HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP was stained with Mouse Anti-Human IgG Fc Monoclonal Antibody (Catalog # MAB110) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human IgG Fc Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Recombinant Human B7‑H1/PD‑L1 Fc Chimera (Catalog # 156-B7) bound to HEK293 human embryonic kidney cell line transfected with human PD-1 and eGFP

Western Blot

1 µg/mL
Sample: Recombinant Human IgG Fc region under non-reducing conditions only

Reviewed Applications

Read 3 reviews rated 4.7 using MAB110 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IgG

Human IgG Fc Antibody Monoclonal Mouse IgG1 Clone # 97924

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Human IgG Fc Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IgG Fc Antibody

For research use only

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