Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Mouse, Rat, Avian - Chicken, Rabbit, Transgenic Mouse

Applications

Validated:

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Cited:

Cell Culture, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunopanning, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # O4

View all Oligodendrocyte Marker O4 Antibodies »

Product Specifications

Immunogen

Bovine brain corpus callosum white matter

Specificity

Detects human, mouse, rat, and chicken Oligodendrocyte Marker O4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Antibody

Detection of Oligodendrocyte Marker O4 antibody in Differentiated Rat Cortical Stem Cells antibody by Flow Cytometry.

Detection of Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells by Flow Cytometry.

Rat cortical stem cells (Catalog # NSC001) either differentiated (filled histogram) or undifferentiated (open histogram) were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326), followed by PE-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116). View our protocol for Staining Membrane-associated Proteins.
Oligodendrocyte Marker O4 antibody in Differentiated Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Oligodendrocyte Marker O4 in Differentiated Rat Cortical Stem Cells.

Oligodendrocyte Marker O4 was detected in immersion fixed differentiated rat cortical stem cells using 1 µg/mL Mouse Anti-Human/Mouse/ Rat/Chicken Oligodendrocyte Marker O4 Monoclonal Antibody (Catalog # MAB1326) for 3 hours at room temperature. Cells were stained (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Olig2 and Oligodendrocyte Marker O4 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells.

Olig2 and Oligodendrocyte Marker O4 were detected in 7 day differentiated rat cortical stem cells using 10 µg/mL Human Olig2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2418) and 10 µg/mL Mouse Anti-Human/Mouse/Rat/Chicken O4 Monoclonal Antibody (Catalog # MAB1326). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Olig2 using the NorthernLights™ 637-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL002), and stained for O4 using an anti-mouse IgM secondary antibody (pseudo-stained green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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Applications for Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Differentiated rat cortical stem cells

Immunocytochemistry

1-10 µg/mL
Sample: Immersion fixed 7 day differentiated rat cortical stem cells
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.7 using MAB1326 in the following applications:

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oligodendrocyte Marker O4

Oligodendrocytes are myelinating cells in the central nervous system (CNS) and form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O4 is an antigen on the surface of oligodendrocyte progenitors (1, 2). It has been commonly used as the earliest recognized marker specific for the oligodendroglial lineage (3-8).

References

  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Bansal, R. and Pfeiffer, S.E. (1989) Proc. Natl. Acad. Sci. USA 86:6181.
  4. Gard, A. et al. (1995) Dev. Biol. 167:596.
  5. Reynolds, R. and Hardy, R. (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Pang, Y. et al. (2000) J. Neurosci. Res. 62:510.
  8. Cai, Z. et al. (2001) Brain Res. 898:126.

Additional Oligodendrocyte Marker O4 Products

Product Documents for Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat/Chicken Oligodendrocyte Marker O4 Antibody

For research use only

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