Human/Mouse/Rat NF-L Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2216
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 268014
Product Specifications
Immunogen
E. coli-derived recombinant human NF-L
Met1-Asp338
Accession # P07196
Met1-Asp338
Accession # P07196
Specificity
Detects human NF-L in direct ELISAs and human, mouse and rat NF-L in Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human (rh) NF‑H or rhNF‑M is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat NF-L Antibody
Detection of Mouse and Rat NF-L by Western Blot.
Western blot shows lysates of mouse brain (hypothalamus) and rat brain (hippocampus). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat NF-L Monoclonal Antibody (Catalog # MAB2216) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018) . A specific band was detected for NF-L at approximately 74 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
NF-L in Human Spinal Cord.
NF-L was detected in immersion fixed paraffin-embedded sections of human spinal cord using 25 µg/mL Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB2216) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; CTS002) and counter-stained with hematoxylin (blue). Specific labeling was localized to the cytoplasm in motor neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
NF‑L in Human Brain.
NF-L was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Mouse Anti-Human NF-L Monoclonal Antibody (Catalog # MAB2216) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Mouse/Rat NF-L Antibody
Application
Recommended Usage
Immunocytochemistry
8 µg/mL
Sample: Immersion fixed U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative)
Sample: Immersion fixed U251 human myeloma cell line (positive) and K562 human chronic myelogenous leukemia cell line (negative)
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord and immersion fixed paraffin-embedded sections of human brain (substantia nigra)
Sample: Immersion fixed paraffin-embedded sections of human spinal cord and immersion fixed paraffin-embedded sections of human brain (substantia nigra)
Western Blot
1 µg/mL
Sample: Mouse brain (hypothalamus) and rat brain (hippocampus)
Sample: Mouse brain (hypothalamus) and rat brain (hippocampus)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NF-L
Long Name
Neurofilament Protein, Light Chain
Alternate Names
CMT2E, NEFL, NF68, NFL
Entrez Gene IDs
4747 (Human)
Gene Symbol
NEFL
UniProt
Additional NF-L Products
Product Documents for Human/Mouse/Rat NF-L Antibody
Product Specific Notices for Human/Mouse/Rat NF-L Antibody
For research use only
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