Detects human PDGF R beta in Western blots. Specificity has been confirmed in binding studies using several different cell lines (1, 2) and by its ability to immunoprecipitate PDGF receptor beta-subunit complexed with 125I‑PDGF-BB (3). It does not recognize the PDGF receptor alpha‑subunit. Detects the PDGF receptor beta-subunit of human and primate species (monkey and baboon) but not the rat or mouse receptors. Its ability to bind to receptors from other species has not been tested.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human PDGF R beta Fluorescein-conjugated Antibody

Detection of PDGF R beta in MG‑63 Human Cell Line by Flow Cytometry.

MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human PDGF R beta Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1263F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human PDGF R beta Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: MG‑63 human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDGF R beta

PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF‑B chains. The PDGF-AA, PDGF‑BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immuno‑globulinlike domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero‑dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist (4).

References

Hart et al. (1987) J. Biol. Chem. 262:10780.
Gronwald et al. (1988) Proc. Natl. Acad. Sci. 85:3435.
Seifert et al. (1989) J. Biol. Chem. 264:8771.
Heldin, C.H. and L. Claesson-Welsh (1994) in Guidebook to Cytokines and Their Receptors, Nicola, N.A. ed. Oxford University Press, New York, p. 202.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Entrez Gene IDs

5159 (Human); 18596 (Mouse); 24629 (Rat)

Gene Symbol

PDGFRB

Additional PDGF R beta Products

Product Documents for Human PDGF R beta Fluorescein-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human PDGF R beta Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human PDGF R beta Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1263F

Key Product Details

Species Reactivity

Validated:

Cited:

Applications