Human/Primate MMP-9 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB936
Key Product Details
Species Reactivity
Validated:
Human, Primate
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair), Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
ELISA Capture, ELISA Development (Capture), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 36020
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human MMP-9
Specificity
Detects human MMP-9 in Western blots. Does not react with the C-terminal truncated form (65 kDa) of recombinant human (rh) MMP-9. In Western blots, approximately 5% cross-reactivity with rhMMP-2 and no cross-reactivity with rhMMP-1 or rhMMP-3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Primate MMP-9 Antibody
MMP‑9 in SW1353 Human Cell Line.
MMP-9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
MMP‑9 in Human Lung.
MMP-9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.Applications for Human/Primate MMP-9 Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed SW1353 human chondrosarcoma cell line
Sample: Immersion fixed SW1353 human chondrosarcoma cell line
Immunohistochemistry
8-25 µg/mL
Sample:
Sample:
Immersion fixed paraffin-embedded sections of human lung
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-9 (Catalog # 911-MP), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human MMP-9 (Catalog # 911-MP), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human MMP-9 Western Blot Standard (Catalog # WBC018)
Sample: Recombinant Human MMP-9 Western Blot Standard (Catalog # WBC018)
Human/Primate MMP-9 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 4.3 using MAB936 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MMP-9
Pro Matrix Metalloproteinase 9 (Pro-MMP-9) is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9, which can degrade collagens and elastin as well as several non-ECM molecules.
Long Name
Matrix Metalloproteinase 9
Alternate Names
CLG4B, Gelatinase B, GELB, MANDP2, MMP9
Gene Symbol
MMP9
Additional MMP-9 Products
Product Documents for Human/Primate MMP-9 Antibody
Product Specific Notices for Human/Primate MMP-9 Antibody
For research use only
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