Brodie, T.M. et al. (2018) Cytometry Part A. 93: 406. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human TIMP-3 (Catalog # 973-TM), see our available Western blot detection antibodies

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: MDA-MBA-231 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-3

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE (1). TIMP-3 also uniquely shows high affinity for binding to the extracellular matrix (2). Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.

References

Amour, A. et al. (1998) FEBS Lett. 435:39.
Leco, K.J. et al. (1994) J. Biol. Chem. 269:9352.

Long Name

Tissue Inhibitors of Metalloproteinases 3

Alternate Names

TIMP3

Entrez Gene IDs

7078 (Human); 21859 (Mouse); 25358 (Rat)

Gene Symbol

TIMP3

UniProt

P35625

Additional TIMP-3 Products

Product Documents for Human TIMP-3 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human TIMP-3 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human TIMP-3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9731

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all TIMP-3 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Applications for Human TIMP-3 Antibody

CyTOF-reported