Human TIMP-3 APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # IC9731A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Intracellular Staining by Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 277128
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-3
Cys24-Pro211
Accession # P35625
Cys24-Pro211
Accession # P35625
Specificity
Detects human TIMP-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TIMP-1, -2, or -4 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human TIMP-3 APC-conjugated Antibody
Detection of TIMP‑3 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.
MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TIMP-3 APC-conjugated Monoclonal Antibody (Catalog # IC9731A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Intracellular Molecules.Applications for Human TIMP-3 APC-conjugated Antibody
Application
Recommended Usage
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Sample: MDA‑MB‑231 human breast cancer cell line
Sample: MDA‑MB‑231 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: TIMP-3
Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE.
TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix. Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
UniProt
Additional TIMP-3 Products
Product Documents for Human TIMP-3 APC-conjugated Antibody
Product Specific Notices for Human TIMP-3 APC-conjugated Antibody
For research use only
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