Human TIMP-3 Biotinylated Antibody
R&D Systems, part of Bio-Techne | Catalog # BAM9731
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Detection (Matched Antibody Pair)
Cited:
ELISA Detection
Label
Biotin
Antibody Source
Monoclonal Mouse IgG2A Clone # 277128
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human TIMP-3
Cys24-Pro211
Accession # P35625
Cys24-Pro211
Accession # P35625
Specificity
Detects human TIMP-3 in ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human TIMP-3 Biotinylated Antibody
Application
Recommended Usage
Human TIMP-3 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIMP-3
Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE (1). TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix (2). Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.
References
- Amour, A. et al. (1998) FEBS Lett. 435:39.
- Leco, K.J. et al. (1994) J. Biol. Chem. 269:9352.
Long Name
Tissue Inhibitors of Metalloproteinases 3
Alternate Names
TIMP3
Gene Symbol
TIMP3
UniProt
Additional TIMP-3 Products
Product Documents for Human TIMP-3 Biotinylated Antibody
Product Specific Notices for Human TIMP-3 Biotinylated Antibody
For research use only
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