IGF-II R/IGF2R Antibody (MEM-238) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-464

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-238

Format

BSA Free

Concentration

1.0 mg/ml

View all IGF-II R/IGF2R Antibodies »

Product Specifications

Immunogen

Recombinant Vaccinia virus encoding CD222.

Epitope

Recognizes an epitope between domains 2 and 5.

Specificity

The antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 (IGF2 receptor), a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal ompartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

Marker

Late Endosome Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for IGF-II R/IGF2R Antibody (MEM-238) - BSA Free

Western Blot: IGF-II R/IGF2R Antibody (MEM-238)BSA Free [NB500-464]

Western Blot: IGF-II R/IGF2R Antibody (MEM-238)BSA Free [NB500-464]

Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).
Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-238) - BSA Free [NB500-464]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-238) - BSA Free [NB500-464]

Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [FITC] [NB100-63599] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238)[NB100-63599]- Staining human peripheral blood monocytes following permeablisation with Leucoperm (Fix & Perm) (BUF09). Image using the FITC form of this antibody.
Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-238) - BSA Free [NB500-464]

Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-238) - BSA Free [NB500-464]

Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Intracellular staining of human peripheral blood with anti-CD222 (MEM-238) PE.
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Applications for IGF-II R/IGF2R Antibody (MEM-238) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-6 ug/ml

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
Western Blot - Non-reducing conditions. This antibody is CyTOF ready.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IGF-II R/IGF2R

CD222 (CIMPR, cation-independent mannose 6-phosphate receptor; IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-beta, last but not least CD222 serves as a receptor for heparanase and even for Listeria.

Long Name

Insulin-like Growth Factor II Receptor

Alternate Names

CD222, CIMPR, IGF-IIR, IGF2R, IGFIIR, M6P-R, MPRI

Entrez Gene IDs

3482 (Human)

Gene Symbol

IGF2R

Additional IGF-II R/IGF2R Products

Product Documents for IGF-II R/IGF2R Antibody (MEM-238) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for IGF-II R/IGF2R Antibody (MEM-238) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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