PDGF R beta [p Tyr1021] Antibody (JE48-54)

Western Blot: PDGF R beta [p Tyr1021] Antibody (JE48-54) [NBP3-32786]

Western Blot: PDGF R beta [p Tyr1021] Antibody (JE48-54) [NBP3-32786] - Western blot analysis of PDGF R beta on different lysates with Rabbit anti-PDGF R beta antibody (NBP3-32786) at 1/1,000 dilution.

Lane 1: NIH/3T3 whole cell lysate
Lane 2: NIH/3T3 treated with 100 ng/mL PDGF for 5 minutes whole cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 124 kDa
Observed band size: 190 kDa

Exposure time: 1 minute;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32786) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.