PDGF R beta Antibody (OTI1E8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-73304

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all PDGF R beta Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human PDGFRB(NP_002600) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PDGF R beta Antibody (OTI1E8) - Azide and BSA Free

Western Blot: PDGF R beta Antibody (OTI1E8)Azide and BSA Free [NBP2-73304]

Western Blot: PDGF R beta Antibody (OTI1E8)Azide and BSA Free [NBP2-73304]

Western Blot: PDGF R beta Antibody (OTI1E8) - Azide and BSA Free [NBP2-73304] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDGFRB .
Immunocytochemistry/ Immunofluorescence: PDGF R beta Antibody (OTI1E8) - Azide and BSA Free [NBP2-73304]

Immunocytochemistry/ Immunofluorescence: PDGF R beta Antibody (OTI1E8) - Azide and BSA Free [NBP2-73304]

Immunocytochemistry/Immunofluorescence: PDGF R beta Antibody (OTI1E8) - Azide and BSA Free [NBP2-73304] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY PDG F beta.

Applications for PDGF R beta Antibody (OTI1E8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PDGF R beta

Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-alpha and PDGF receptor-beta. PDGF receptor-beta can only bind two specific PDGF isoforms, PDGF-beta and PDGF-delta (1,2). Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase (3), Grb2, and Src, among others.

Long Name

Platelet-derived Growth Factor Receptor beta

Alternate Names

CD140b, PDGFRB

Gene Symbol

PDGFRB

Additional PDGF R beta Products

Product Documents for PDGF R beta Antibody (OTI1E8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for PDGF R beta Antibody (OTI1E8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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