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N-2 Plus Media Supplement

R&D Systems, part of Bio-Techne | Catalog # AR003

R&D Systems, part of Bio-Techne
Neural Progenitor Cells Expanded with N-2 Plus Media Supplement Express Nestin and SOX2
(2)

Key Product Details

Assay Procedure

Refer to the product datasheet for complete product details.

Briefly, completed N-2 Plus-supplemented neural cell medium is prepared using the following procedure:

  • Dilute the media supplement in basal media
  • Store completed media
  • Use within 2 weeks
 

 

Reagents Provided

Reagents provided in the N-2 Plus Media Supplement (Catalog # AR003):

  • Bovine Insulin (2,500 µg/mL)
  • Human Transferrin (10,000 µg/mL)
  • Putrescine (1,611 µg/mL)
  • Selenite (0.52 µg/mL)
  • Progesterone (0.63 µg/mL)

 

Other Supplies Required

Reagents

  • DMEM/F-12 (Invitrogen®, Catalog # 12500-062) or a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029)
  • Glucose
  • Glutamine
  • NaHCO3
  • Penicillin-Streptomycin (100X)
  • Deionized or distilled water

Materials

  • Serological pipettes
  • Pipettes and pipette tips
  • 2 µm filter unit

Equipment

  • 2 °C to 8 °C refrigerator

 

Procedure Overview

Option 1: Mix the following components with deionized or distilled water to make 500 mL of medium.

Component Amount
DMEM/F-12 6 g
Glucose 0.775 g
Glutamine 0.0365 g
NaHCO3 0.854 g
N-2 Plus Media Supplement 5 mL

Adjust the pH to 7.2. Filter the solution (2 µm filter unit), and add 5 mL of 100X sterile Penicillin-Streptomycin solution. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

Option 2: Dilute 100-fold with a basal media (e.g., Neurobasal Media from Invitrogen, Catalog # 21103-029) before use. The medium may be stored in the dark at 2 °C to 8 °C for up to 2 weeks.

 

Invitrogen is a registered trademark of Invitrogen Corp.

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Product Documents for N-2 Plus Media Supplement

Certificate of Analysis

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