LDN 193189 dihydrochloride

Name: LDN 193189 dihydrochloride
Brand: Tocris Bioscience
SKU: 6053
Rating: 4 (1 reviews)

Tocris Bioscience, part of Bio-Techne | Catalog # 6053

(3)

Citations (74)

COA / SDS

Potent and selective ALK2 and ALK3 inhibitor; inhibits BMP4 signaling; promotes neural induction of hPSCs

Key Product Details

Chemical Name:	4-[6-[4-(1-Piperazinyl)phenyl]pyrazolo[1,5-a]pyrimidin-3-yl]quinoline dihydrochloride
Purity:	≥98%

View Full Technical Details for LDN 193189 dihydrochloride

Technical Data
Citations / Reviews (3)

Biological Activity for LDN 193189 dihydrochloride

LDN 193189 dihydrochloride is a potent and selective ALK2 and ALK3 inhibitor (IC₅₀ values are 5 and 30 nM, respectively); inhibits BMP4-mediated Smad1/5/8 activation. Exhibits >200-fold selectivity for BMP signaling over TGF-β signaling. Also exhibits selectivity over AMPK, PDGFR and MAPK signaling. Promotes neural induction of hPSCs in combination with SB 431542 (Cat.No. 1614). Also induces differentiation of hPSCs into nociceptive sensory neurons in combination with SB 431542 (Cat.No. 1614), SU 5402 (Cat.No.3300), CHIR 99021 (Cat.No. 4423) and DAPT (Cat.No. 2634).

LDN 193189 synthesized to cGMP guidelines and LDN 193189 in solution also available.

For more information about how LDN 193189 dihydrochloride may be used, see our protocols: Generation of β cells from hPSCs, Generating Midbrain Dopaminergic Neurons from hPSCs, Accelerated Induction of Cortical Neurons from hiPSCs.

Scientific Data Examples for LDN 193189 dihydrochloride

	Application of LDN 193189 in motor neurons differentiated from iPSCs. Image shows IBJ6 human fibroblast induced pluripotent stem cells which have been differentiated into motor neurons. This was performed using a combination of LDN 193189 (100 nM) and SB 431542 (Catalog # 1614, Tocris) followed by Retinoic acid (Catalog # 0695, Tocris) and Purmorphamine (Catalog # 4551, Tocris) culture on Laminin for 18 days. Commitment to the motor neuron fate was assessed using the motor neuron marker anti-Human Islet-1 (red, R&D Systems, Catalog # AF1837) and the general neuronal marker, beta III tubulin (green, R&D Systems, Catalog # MAB1195). For visualization, the neurons were stained using Northernlights™ 557-conjugated Donkey anti-Goat Secondary Antibody (R&D Systems, Catalog # NL001) and Northernlights™ 493-conjugated Donkey anti-Mouse Secondary Antibody (R&D Systems, Catalog # NL009 NL009) and counterstained with DAPI (blue, Catalog # 5748, Tocris).

Technical Data for LDN 193189 dihydrochloride

Formula	C₂₅H₂₂N₆.2HCl
Storage	Store at -20°C
Purity	≥98%
CAS Number	1435934-00-1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for LDN 193189 dihydrochloride

Solubility

Soluble Soluble to 50 mM in water and to 10 mM in DMSO

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References for LDN 193189 dihydrochloride

References are publications that support the biological activity of the product.

N Pode-Shakk, R Gershon, G Tam, D Omer, Y Gnatek, I Kanter, S Oriel, G Katz, O Harari-Ste, T Kalisky, B Dekel Evidence of Inï¿½Vitro Preservation of Human Nephrogenesis at the Single-Cell Level Stem Cell Reports, 2017;0(0):. 2017 PMID: 28552604
Yu et al. BMP type I receptor inhibition reduces heterotopic ossification. Nat.Med., 2008;14:1363 PMID: 19029982
Chambers et al. Combined small-molecule inhibition accelerates developmental timing and converts human pluripotent stem cells into nociceptors. Nat.Biotechnol., 2012;30:715 PMID: 22750882
Cuny et al. Structure-activity relationship study of bone morphogenetic protein (BMP) signaling inhibitors. Bioorg.Med.Chem.Lett., 2008;18:4388 PMID: 18621530
Li et al. Chemical approaches to stem cell biology and therapeutics. Cell Stem Cell, 2013;13:270 PMID: 24012368
Lancaster et al. Generation of Cerebral Organoids from Human Pluripotent Stem Cells Nat.Protoc., 2015;9:2329 PMID: 25188634
Dye et al. In vitro generation of human pluripotent stem cell derived lung organoids. Elife, 2015; PMID: 25803487
Wimmer et al. Human blood vessel organoids as a model of diabetic vasculopathy. Nature, 2019;565:505 PMID: 30651639
Kadoshima et al. Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell-derived neocortex. Proc.Natl.Acad.Sci.USA., 2013;110:20284 PMID: 24277810